肌醇三磷酸从融合性胡萝卜（Daucus carota L.）原生质体中动员钙。
myo-Inositol Trisphosphate Mobilizes Calcium from Fusogenic Carrot (Daucus carota L.) Protoplasts.
机构信息
Department of Botany, North Carolina State University, Raleigh, North Carolina 27695.
出版信息
Plant Physiol. 1987 Feb;83(2):395-8. doi: 10.1104/pp.83.2.395.
Abstract
To determine whether or not inositol trisphosphate (IP(3)) mobilizes calcium in higher plant cells, we investigated the effect of IP(3) on Ca(2+) fluxes in fusogenic carrot (Daucus carota L.) protoplasts. The protoplasts were incubated in (45)Ca(2+)-containing medium and the (45)Ca(2+) associated with the protoplasts was monitored with time. Addition of IP(3) (20 micromolar) caused a 17% net loss of the accumulated (45)Ca(2+) within 4 minutes. There was a reuptake of (45)Ca(2+) and the protoplasts recovered to their initial value by 10 minutes. Phytic acid (IP(6)), also stimulated (45)Ca(2+) efflux from the protoplasts. Both the IP(3(-) ) and the IP(6(-) )induced (45)Ca(2+) efflux were inhibited by the calmodulin antagonist, trifluoperazine.
摘要
为了确定三磷酸肌醇（IP（3））是否动员高等植物细胞中的钙，我们研究了 IP（3）对融合性胡萝卜（Daucus carota L.）原生质体中 Ca（2+）流的影响。将原生质体在含有（45）Ca（2+）的培养基中孵育，并随时间监测与原生质体相关的（45）Ca（2+）。添加 IP（3）（20 微摩尔）在 4 分钟内导致累积的（45）Ca（2+）净损失 17%。（45）Ca（2+）被再摄取，并且原生质体在 10 分钟内恢复到初始值。植酸（IP（6））也刺激原生质体中（45）Ca（2+）的流出。IP（3（-））和 IP（6（-））诱导的（45）Ca（2+）流出均被钙调蛋白拮抗剂三氟拉嗪抑制。
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本文引用的文献
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