Ege University Faculty of Pharmacy, Dep. of Pharmaceutical Microbiology, Izmir, Türkiye.
lzmir Katip Celebi University, Atatürk Training and Research Hospital Medical Microbiology Laboratory, Izmir, Türkiye.
Pol J Microbiol. 2013;62(3):295-8.
The present study aimed to evaluate antimicrobial activity of tigecydcline against 84 multidrug resistant (MDR) Acinetobacter spp. strains by disc diffusion and E-test methods. The results of disc diffusion test were compared according to two different interpretation ways. In addition, E-test results and the disc diffusion results that interpreted by both the methods were checked for compatibility. According to the disc diffusion test, 3 strains (3.57%) were found resistant to tigecycline when considering breakpoints suggested by Food and Drug Administration (FDA). On the other hand, none of the strains was found resistant to the evaluation criteria recommended by Jones etal. (2007). Considering E-test results of tigecycline, MIC, and MICG, values of tigecycline for Acinetobacter spp. were 0.75 and 1 mg/l, respectively. Based on FDA defined breakpoints for Enterobacteriaceae, any resistant isolate was detected. In conclusion, although there are some differences in the results, tigecycline was found quite effective on Acinetobacter spp. isolates with reference to the both disc diffusion and the E-test methods.
本研究旨在通过纸片扩散和 E 试验方法评估替加环素对 84 株多药耐药（MDR）不动杆菌属菌株的抗菌活性。根据两种不同的解释方法比较了纸片扩散试验的结果。此外，还检查了 E 试验结果和两种方法解释的纸片扩散结果的兼容性。根据纸片扩散试验，当考虑到食品和药物管理局（FDA）建议的折点时，发现 3 株（3.57%）菌株对替加环素耐药。另一方面，根据 Jones 等人推荐的评估标准（2007 年），没有一株对替加环素耐药。考虑替加环素的 E 试验结果、MIC 和 MICG 值，替加环素对不动杆菌属的 MIC 值分别为 0.75 和 1mg/L。根据 FDA 定义的肠杆菌科折点，检测到任何耐药分离株。总之，尽管结果存在一些差异，但参照纸片扩散和 E 试验方法，替加环素对不动杆菌属分离株的疗效相当显著。
