[2型糖尿病患者尿液生物标志物的筛查]
[Screening of urinary biomarkers in patients with type 2 diabetes mellitus].
作者信息
Fu Han, Liu Xiaoli, Yu Wei, Zhu Lijia, Zheng Dan, Wang Jun
机构信息
MOE Key Lab of Environment and Health, Institute of Environmental Medicine, School of Public Health, Tongji Medical College, Huazhong University of Science, Wuhan 430030, China.
出版信息
Wei Sheng Yan Jiu. 2013 Nov;42(6):907-14.
OBJECTIVE
To screen the biomarkers of the middle-aged type 2 diabetes (T2DM) in urine based on metabolomics.
METHODS
First, both the metabolite profiles of morning urine sample in T2DM and control group were obtained by liquid chromatography-mass spectrometry (LC-MS). Second, an automated compound (feature) extraction algorithm was employed for processing background subtracted single MS data. Then principal component analysis (PCA) and t-Test analysis was followed after preprocessing on the extractive data. Third, identifying the potential biomarkers that show significant difference between the two groups by searching the database online and comparing the results of MS/MS experiments to those available in literature and those standards.
RESULTS
Three endogenous metabolites, among which tryptophan, kynurenic acid, and shown down-regulation while kynurenine shown up-regulation in T2DM, significantly differentiate between T2DM and control group.
CONCLUSION
Tryptophan, kynurenic acid and kynurenine may be considered as the potentail biomarkers related to T2DM.
目的
基于代谢组学筛选中年2型糖尿病（T2DM）患者尿液中的生物标志物。
方法
首先，通过液相色谱-质谱联用（LC-MS）获得T2DM组和对照组晨尿样本的代谢物谱。其次，采用自动化合物（特征）提取算法处理扣除背景后的单重质谱数据。然后，对提取的数据进行预处理后进行主成分分析（PCA）和t检验分析。第三，通过在线搜索数据库，并将MS/MS实验结果与文献中的结果和标准进行比较，确定两组之间存在显著差异的潜在生物标志物。
结果
三种内源性代谢物在T2DM组与对照组之间有显著差异，其中色氨酸、犬尿酸呈下调，而犬尿喹啉酸呈上调。
结论
色氨酸、犬尿酸和犬尿喹啉酸可能被视为与T2DM相关的潜在生物标志物。
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