Wang He, Yu Jiyun, Li Li
Tumor Hospital of Guangxi Medical University, Nanning 530021, China.
Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2013 Oct;30(5):1102-7.
To construct and express a composite gene vaccine for human papillomavirus 58(HPV58)-associated cervical cancer, we inserted HPV58mE6E7 fusion gene into pCI-Fc-GPI eukaryotic expression vector, constructing a recombinant plasmid named pCI-sig-HPV58mE6E7-Fc-GPI. Then we further inserted fragment of sig-HPV58mE6E7Fc-GPI into the novel vaccine vector PVAX1-IRES-GM/B7, constructing PVAX1-HPV58mE6E7FcGB composite gene vaccine. PVAX1-HPV58mE6E7FcGB vaccine was successfully constructed and identified by restriction endonuclease and sequencing analysis. Eukaryotic expression of fusion antigen sig-HPV58mE6E7-Fc-GPI and molecular ad-juvant GM-CSF and B7. 1 were proved to be realized at the same time by flow cytometry and immunofluorescence. So PVAX1-HPV58mE6E7FcGB can be taken as a candidate of therapeutic vaccine for HPV58-associated tumors and their precancerous transformations.
为构建并表达用于人乳头瘤病毒58型（HPV58）相关宫颈癌的复合基因疫苗，我们将HPV58mE6E7融合基因插入pCI-Fc-GPI真核表达载体，构建了名为pCI-sig-HPV58mE6E7-Fc-GPI的重组质粒。然后我们将sig-HPV58mE6E7Fc-GPI片段进一步插入新型疫苗载体PVAX1-IRES-GM/B7，构建了PVAX1-HPV58mE6E7FcGB复合基因疫苗。通过限制性内切酶和测序分析成功构建并鉴定了PVAX1-HPV58mE6E7FcGB疫苗。通过流式细胞术和免疫荧光证明，融合抗原sig-HPV58mE6E7-Fc-GPI以及分子佐剂GM-CSF和B7.1的真核表达同时得以实现。因此，PVAX1-HPV58mE6E7FcGB可作为HPV58相关肿瘤及其癌前病变的治疗性疫苗候选物。
