Su Song
Department of Hepatobiliary Surgery, the First Affiliated Hospital of Luzhou Medical College, Luzhou 646000, China.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2013 Oct;30(5):1108-11.
This paper is aimed to establish a novel abdominal heart transplantation model in mice and to generalize the experience of the successful cases. The thoracic inferior vena cava instead of pulmonary artery was employed to reconstruct the outflow tract of the graft heart (in the new method group, 82 cases). Meanwhile, in other 47 cases as the control group, traditional anastomosis was used between pulmonary artery of the graft and vena cava of the recipient. The recipient surgery time, vena cava-vena cava anastomosis time, graft cold ischemia time and graft re-beating time were (41.5 +/- 1. 5) min, (8.4 +/- 0.6) min, (32.3 +/- 0.4) min and (1.5 +/- 0.2) min respectively. All the above data were statistically superior to those in the traditional method group (P < 0.001 or P < 0.05). The survival rate of 100 d post surgery in the new method group was 93. 9%. Meanwhile, the cardiac tissue remained almost normal examined by HE and Picro-sirus red staining. Therefore, the novel model can facilitate the anastomosis of the outflow tract in recipient operation in mouse heart transplantation model.
本文旨在建立一种新型小鼠腹部心脏移植模型，并推广成功案例的经验。采用胸段下腔静脉而非肺动脉来重建移植心脏的流出道（新方法组，82例）。同时，在另外47例作为对照组的实验中，采用传统方法将移植心脏的肺动脉与受体的腔静脉进行吻合。受体手术时间、腔静脉-腔静脉吻合时间、移植心脏冷缺血时间和移植心脏复跳时间分别为（41.5±1.5）分钟、（8.4±0.6）分钟、（32.3±0.4）分钟和（1.5±0.2）分钟。上述所有数据在统计学上均优于传统方法组（P<0.001或P<0.05）。新方法组术后100天的生存率为93.9%。同时，通过苏木精-伊红（HE）染色和苦味酸天狼星红染色检查发现心脏组织几乎保持正常。因此，该新型模型有助于在小鼠心脏移植模型的受体手术中进行流出道的吻合。
