Dept. of Food Science and Agriculture, Dept. of Poultry Science, and Faculty of College of Agriculture, Auburn Univ., Auburn, Ala. 36849, U.S.A.
J Food Sci. 2014 Jan;79(1):M54-60. doi: 10.1111/1750-3841.12220.
The objective of this study was to evaluate anti-listerial efficacy of salts of organic acids, and their impact on the quality of frankfurters. Beef frankfurters were manufactured by incorporating organic acids in 5 different combinations: (1) control (no marinade addition; C); (2) sodium lactate (2% wt/wt; SL); (3) potassium lactate (2% wt/wt; PL); (4) sodium citrate (0.75% wt/wt; SC); and (5) sodium lactate (2% wt/wt)/sodium diacetate (0.25% wt/wt; SL/SD). Cooked frankfurters were inoculated with streptomycin-resistant (1500 μg/mL) L. monocytogenes (7 log₁₀ CFU/frank). Inoculated and noninoculated frankfurters were vacuum packaged and stored at 4 °C. Samples were taken weekly up to 10 wk for estimation of L. monocytogenes as well as aerobic plate count (APC) and psychrotrophs (PSY), respectively. Total of 2 independent trials of the entire experiment were conducted. Noninoculated beef frankfurters were evaluated weekly by untrained sensory panelists for 7 wk. SL, PL, and SC treatments did not (P > 0.05) adversely affect consumer acceptability through 8 wk although, SL/SD treatment was significantly (P ≤ 0.05) less preferred across all sensory attributes. SL/SD treatment negatively affected product quality, but was able to control APC, PSY, and L. monocytogenes levels. SC performed similar to the control throughout the 8, 9, and 10 wk storage periods, providing no benefit for inhibiting L. monocytogenes (increasing from 7 logs CFU/frank to 10 logs CFU/frank throughout storage) or extending shelf life of the beef frankfurters. In conclusion, 2% SL and PL, and 2% SL/0.25% SD may be effective L. monocytogenes inhibitors (maintaining inoculation levels of 7 logs CFU/frank during storage), but changes in SL/SD treatment formulation should be studied to improve product quality.
本研究旨在评估有机酸盐的抗李斯特菌效果及其对法兰克福香肠质量的影响。通过在 5 种不同组合中添加有机酸来制作牛肉法兰克福香肠:(1)对照(无腌制添加;C);(2)乳酸钠(2%wt/wt;SL);(3)乳酸钾(2%wt/wt;PL);(4)柠檬酸钠(0.75%wt/wt;SC);和(5)乳酸钠(2%wt/wt)/双乙酸钠(0.25%wt/wt;SL/SD)。将煮熟的法兰克福香肠接种链霉素抗性(1500μg/mL)李斯特菌(7log₁₀CFU/frank)。接种和未接种的法兰克福香肠均采用真空包装,并在 4°C 下储存。每周取样一次,直至 10 周,分别用于估计李斯特菌以及好氧平板计数(APC)和嗜冷菌(PSY)。整个实验共进行了 2 次独立试验。在 7 周内,未接种的牛肉法兰克福香肠每周由未经训练的感官品评小组进行评估。尽管 SL、PL 和 SC 处理没有(P>0.05)通过 8 周的时间对消费者的可接受性产生不利影响,但 SL/SD 处理在所有感官属性方面均明显(P≤0.05)较差。SL/SD 处理会对产品质量产生负面影响,但能够控制 APC、PSY 和李斯特菌水平。SC 在整个 8、9 和 10 周的储存期内表现与对照相似,对抑制李斯特菌(从 7 个对数 CFU/frank 增加到整个储存期间的 10 个对数 CFU/frank)或延长牛肉法兰克福香肠的保质期没有任何益处。总之,2%SL 和 PL,以及 2%SL/0.25%SD 可能是有效的李斯特菌抑制剂(在储存期间保持接种水平为 7 个对数 CFU/frank),但应研究 SL/SD 处理配方的变化,以提高产品质量。