Tirloni Erica, Bernardi Cristian, Fusi Viviana, Sgoifo Rossi Carlo Angelo, Stella Simone
Department of Veterinary Medicine and Animal Sciences, University of Milan, Via Dell'Università 6, I-26020, Lodi, Italy.
Heliyon. 2024 May 8;10(10):e30883. doi: 10.1016/j.heliyon.2024.e30883. eCollection 2024 May 30.
In the present study, growth potential of in steak tartare samples taken at retail and belonging to 13 brands marketed in Northern Italy was investigated. The samples were submitted to microbiological and chemical-physical characterization. The data obtained were used as inputs for the application of the predictive microbiology software FSSP that allows the estimation of the growth of during the shelf-life. Lactic acid bacteria, the main component of the microflora, gave variable counts among the brands (from 3.38 to 6.24 log CFU/g). pH and a values were always higher than 5.3 and 0.96, respectively, thus they could not be considered as single efficient hurdles to prevent the growth of according to the EC Reg. 2073/2005; the same was observed for salt content (constantly <2 %) and nitrites (not quantifiable in all the samples, even if declared in some labels). Nevertheless, the combination of all the hurdles, evaluated by predictive microbiology using critical development factors, resulted in an estimated growth <0.5 log CFU/g throughout the shelf life; this output allowed us to consider all the steak tartare analysed as unfavourable substrate for growth. The information obtained could be useful for tartare producers as well as for competent authority to evaluate the effective risk concerning these typology of products.
在本研究中,对意大利北部市场上13个品牌的零售鞑靼牛排样品中[具体微生物名称未给出]的生长潜力进行了调查。对这些样品进行了微生物学和化学物理特性分析。所获得的数据被用作预测微生物学软件FSSP的输入,该软件可估计[具体微生物名称未给出]在保质期内的生长情况。作为微生物群落主要成分的乳酸菌,在不同品牌中的计数有所不同(从3.38到6.24 log CFU/g)。pH值和a值始终分别高于5.3和0.96,因此根据欧盟法规2073/2005,它们不能被视为阻止[具体微生物名称未给出]生长的单一有效障碍;盐含量(始终<2%)和亚硝酸盐(在所有样品中均无法定量,即使在某些标签中声明有)的情况也是如此。然而,通过使用关键生长因子的预测微生物学评估,所有障碍因素的组合导致在整个保质期内估计生长量<0.5 log CFU/g;这一结果使我们能够将所有分析的鞑靼牛排视为不利于[具体微生物名称未给出]生长的底物。所获得的信息对于鞑靼牛排生产商以及主管当局评估这些类型产品的有效风险可能是有用的。