González Ana M, Arnaiz Ignacio, Yus Eduardo, Eiras Carmen, Sanjuán María, Diéguez Francisco J
ADSG Vacasan, Sobreira-Fornás s/n, 27500 Chantada, Spain.
Laboratorio de Sanidad y Producción Animal de Galicia (LASAPAGA), Av. Madrid 77, 27002 Lugo, Spain.
Vet J. 2014 Mar;199(3):424-8. doi: 10.1016/j.tvjl.2013.12.005. Epub 2013 Dec 11.
The aim of the present study was to determine the serological response of heifers after vaccination with two inactivated bovine viral diarrhoea virus (BVDV) vaccines by means of various ELISA tests. Three dairy farms were selected from the Galicia region of Spain. In each herd, a batch of heifers to be vaccinated for the first time was selected and followed for 15 months. Heifers from farm 1 (n=25) were vaccinated with Vaccine A, whereas heifers from farm 2 (n=16) were vaccinated with Vaccine B. Heifers from farm 3 (n=17), where no BVDV vaccines were used, acted as controls. Blood samples were analyzed periodically for BVDV antibodies, using five commercial ELISAs, based on BVDV p80 antigen or whole virus. At the end of the study, none of the animals vaccinated with Vaccine A seroconverted according to p80 antibody status, whereas up to 80% tested positive by ELISA against whole virus antigen. For the animals vaccinated with Vaccine B, 2/16 animals seroconverted according to p80 antibody ELISAs, whereas all had seroconverted according to the ELISA against whole virus antigen. In most cases, based on the use of ELISAs to detect specific antibodies against the p80 protein, at 15 months post-vaccination with inactivated BVDV vaccines the responses did not seem to interfere with detection of antibody to BVDV infection. However, the finding of a small proportion of vaccinated animals seropositive against BVDV p80 antigen suggests that antibodies that interfere with diagnosis of BVDV infection within the herd could exist, even when using p80 ELISAs.
本研究的目的是通过各种酶联免疫吸附测定(ELISA)试验,确定初产小母牛接种两种灭活牛病毒性腹泻病毒(BVDV)疫苗后的血清学反应。从西班牙加利西亚地区选择了三个奶牛场。在每个牛群中,挑选一批首次接种疫苗的小母牛,并跟踪15个月。农场1的小母牛(n = 25)接种疫苗A,而农场2的小母牛(n = 16)接种疫苗B。农场3的小母牛(n = 17)未使用BVDV疫苗,作为对照。使用基于BVDV p80抗原或全病毒的五种商用ELISA定期分析血样中的BVDV抗体。在研究结束时,根据p80抗体状态,接种疫苗A的动物均未发生血清转化,而高达80%的动物通过针对全病毒抗原的ELISA检测呈阳性。对于接种疫苗B的动物,根据p80抗体ELISA,2/16的动物发生了血清转化,而根据针对全病毒抗原的ELISA,所有动物均发生了血清转化。在大多数情况下,基于使用ELISA检测针对p80蛋白的特异性抗体,在接种灭活BVDV疫苗15个月后,这些反应似乎不会干扰对BVDV感染抗体的检测。然而,一小部分接种疫苗的动物对BVDV p80抗原呈血清阳性这一发现表明,即使使用p80 ELISA,牛群中可能存在干扰BVDV感染诊断的抗体。