Young N J, Thomas C J, Thompson I, Collins M E, Brownlie J
Department of Pathology and Infectious Diseases, The Royal Veterinary College, Hawkshead Lane, North Mymms, Hatfield, Hertfordshire AL9 7TA, UK.
Prev Vet Med. 2005 Nov 15;72(1-2):115-20; discussion 215-9. doi: 10.1016/j.prevetmed.2005.08.013. Epub 2005 Sep 16.
Immune responses to non-structural protein 3 (NS3) of bovine viral diarrhoea virus (BVDV) were investigated. cDNA encoding NS3 from type 1a BVDV was used to vaccinate five calves, another five calves remained unvaccinated. Three weeks after final vaccination animals were challenged intranasally with heterologous type 1a BVDV. Anti-NS3 antibodies were detected in only one animal post-vaccination. Partial protection from virus challenge was observed in the vaccinates. Virus was not isolated from nasal mucosa of two vaccinates, and virus clearance from nasal mucosa was faster in the vaccinates compared to the controls. While elevated rectal temperatures were evident in both groups 7 days post-challenge, the mean increase in the controls was twice that observed in the vaccinates. In conclusion, NS3 DNA vaccination induced humoral immunity in one calf, and prevented fever and virus establishment in the nasal mucosa in 2/5 calves, demonstrating the efficacy of NS3 vaccination, which may benefit future development of pestivirus and flavivirus vaccines.
对牛病毒性腹泻病毒（BVDV）非结构蛋白3（NS3）的免疫反应进行了研究。用编码1a型BVDV NS3的cDNA对5头犊牛进行疫苗接种，另外5头犊牛未接种疫苗。最后一次接种疫苗三周后，用异源1a型BVDV经鼻对动物进行攻毒。接种疫苗后仅在1只动物中检测到抗NS3抗体。在接种疫苗的动物中观察到对病毒攻毒的部分保护作用。在2只接种疫苗的动物的鼻黏膜中未分离到病毒，与对照组相比，接种疫苗的动物鼻黏膜中的病毒清除速度更快。虽然在攻毒后7天两组动物的直肠温度均明显升高，但对照组的平均升高幅度是接种疫苗动物的两倍。总之，NS3 DNA疫苗接种在1头犊牛中诱导了体液免疫，并在2/5的犊牛中预防了发热和鼻黏膜中的病毒定植，证明了NS3疫苗接种的有效性，这可能有利于瘟病毒和黄病毒疫苗的未来开发。
