一种新的干奶采样技术及其在奶牛孕酮检测中的应用。
A new dried milk sampling technique and its application for progesterone detection in cows.
作者信息
Samsonova Jeanne V, Osipov Alexander P, Kondakov Sergey E
机构信息
Faculty of Chemistry, M.V. Lomonosov Moscow State University, 119991 Moscow, Russia.
Faculty of Chemistry, M.V. Lomonosov Moscow State University, 119991 Moscow, Russia.
出版信息
Vet J. 2014 Mar;199(3):471-2. doi: 10.1016/j.tvjl.2013.10.031. Epub 2013 Oct 29.
A new method for milk sample collection and storage, based on a dried milk sampling technique, is proposed. The method includes application of a whole milk sample to a porous membrane followed by drying. One hundred whole milk samples (dried and liquid) taken on day 21 post insemination were analysed for progesterone by ELISA and results for both dried and liquid samples were well correlated (r=0.911). Milk progesterone ELISA accuracy for pregnancy diagnosis in cows was 87%.
本文提出了一种基于干奶采样技术的牛奶样本采集与储存新方法。该方法包括将全脂牛奶样本施加到多孔膜上，然后进行干燥。对授精后第21天采集的100份全脂牛奶样本（干样和液态样）进行了酶联免疫吸附测定（ELISA）孕酮分析，干样和液态样的结果相关性良好（r=0.911）。奶牛妊娠诊断的牛奶孕酮ELISA准确率为87%。
Zotero 插件