From the King Edward VII Hospital (Spalton), London, and the IOL Research Group (Russell, Evans-Gowing, Eldred, Wormstone), School of Biological Sciences, University of East Anglia, Norwich, United Kingdom.
From the King Edward VII Hospital (Spalton), London, and the IOL Research Group (Russell, Evans-Gowing, Eldred, Wormstone), School of Biological Sciences, University of East Anglia, Norwich, United Kingdom.
J Cataract Refract Surg. 2014 Feb;40(2):306-12. doi: 10.1016/j.jcrs.2013.06.030.
To evaluate the effect of complete destruction of lens epithelial cells (LECs) in the capsular bag on intraocular lens (IOL) stability.
School of Biological Sciences, University of East Anglia, Norwich, United Kingdom.
Comparative evaluation.
An in vitro organ culture model using the bag-zonule-ciliary body complex isolated from fellow human donor eyes was prepared. A capsulorhexis and fiber extraction were performed, and an Acrysof IOL was implanted. Preparations were secured by pinning the ciliary body to a silicone ring and maintaining it in 6 mL Eagle minimum essential medium supplemented with 5% v/v fetal calf serum and 10 ng/mL transforming growth factor-β2 for 3 weeks or more. One bag of each pair was treated with 1 μM thapsigargin to destroy all LECs. Observations of LEC growth were captured by phase-contrast microscopy, IOL stability by video microscopy, and endpoint analysis through scanning electron microscopy and immunocytochemistry.
The LECs in control capsular bags migrated centrally, closing the bag and fixating the IOL between the anterior and posterior capsules, as seen clinically. These events were not observed in the thapsigargin-treated group. After a period of controlled orbital movement, the IOL in the control group stabilized quicker than in the treated bags. There was no IOL rotation in the bag; however, the IOLs in the treated group rocked with axial movement.
The LECs appeared to aid stabilization of current IOL designs in the capsular bag. The results have clinical implications for IOL design and for strategies to prevent posterior capsule opacification.
No author has a financial or proprietary interest in any material or method mentioned.
评估在囊袋内完全破坏晶状体上皮细胞（LEC）对人工晶状体（IOL）稳定性的影响。
英国东安格利亚大学生物科学学院。
对比评估。
使用从供体眼分离的囊袋-悬韧带-睫状体复合体建立体外器官培养模型。进行囊膜环形撕除和纤维提取，并植入 Acrysof IOL。通过将睫状体别针固定在硅环上，并将其保持在补充有 5%v/v 胎牛血清和 10ng/mL 转化生长因子-β2 的 6mL Eagle 最小必需培养基中 3 周或更长时间来固定准备工作。每对囊袋中的一个用 1μM 他普西卡丁处理以破坏所有 LEC。通过相差显微镜观察 LEC 生长，通过视频显微镜观察 IOL 稳定性，并通过扫描电子显微镜和免疫细胞化学进行终点分析。
在对照组囊袋中，LEC 向中央迁移，封闭囊袋并将 IOL 固定在前囊和后囊之间，如临床上所见。在他普西卡丁处理组中未观察到这些事件。经过一段时间的受控眼球运动后，对照组的 IOL 比处理组的囊袋更快地稳定下来。在囊袋中没有 IOL 旋转，但在处理组中，IOL 随着轴向运动而晃动。
LEC 似乎有助于稳定当前在囊袋中的 IOL 设计。这些结果对 IOL 设计和预防后囊混浊的策略具有临床意义。
