Jiang Huan, Miao Ming, Ye Fan, Jiang Bo, Zhang Tao
State Key Laboratory of Food Science & Technology, Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu 214122, PR China.
State Key Laboratory of Food Science & Technology, Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu 214122, PR China.
Int J Biol Macromol. 2014 Apr;65:208-14. doi: 10.1016/j.ijbiomac.2014.01.044. Epub 2014 Jan 24.
In this study, partial 4-α-glucanotransferase (4αGT) treatment was used to modulate the fine structure responsible for the slow digestion and resistant property of starch. Normal corn starch modified using 4αGT for 4h showed an increase of slowly digestible starch from 9.40% to 20.92%, and resistant starch from 10.52 to 17.63%, respectively. The 4αGT treatment decreased the content of amylose from 32.6% to 26.8%. The molecular weight distribution and chain length distribution of 4αGT-treated starch showed a reduction of molecular weight and a great number of short (DP<13) and long (DP>30) chains through cleaving and reorganization of starch molecules. Both the short and long chain fractions of modified amylopectin were attributed to the low in vitro digestibility. The viscosity was inversely related to the digestibility of the 4αGT-treated starch. These results suggested that the 4αGT modified starch synthesized the novel amylopectin clusters with slow digestible and resistant character.
在本研究中，使用部分4-α-葡聚糖转移酶（4αGT）处理来调节负责淀粉缓慢消化和抗性特性的精细结构。用4αGT处理4小时的普通玉米淀粉显示，缓慢消化淀粉的含量分别从9.40%增加到20.92%，抗性淀粉从10.52%增加到17.63%。4αGT处理使直链淀粉含量从32.6%降至26.8%。4αGT处理淀粉的分子量分布和链长分布显示，通过淀粉分子的裂解和重组，分子量降低，出现大量短链（DP<13）和长链（DP>30）。改性支链淀粉的短链和长链部分均归因于低体外消化率。粘度与4αGT处理淀粉的消化率呈负相关。这些结果表明，4αGT改性淀粉合成了具有缓慢消化和抗性特征的新型支链淀粉簇。
