降低辅因子有助于野生型梭菌 BOH3 菌株丁醇产量的增加。
Reducing cofactors contribute to the increase of butanol production by a wild-type Clostridium sp. strain BOH3.
机构信息
Department of Civil and Environmental Engineering, National University of Singapore, Singapore 117576, Singapore.
Department of Civil and Environmental Engineering, National University of Singapore, Singapore 117576, Singapore.
出版信息
Bioresour Technol. 2014 Mar;155:220-8. doi: 10.1016/j.biortech.2013.12.089. Epub 2014 Jan 2.
Availability of reducing factors (e.g., NADH and NADPH) plays an important role in improving the efficacy of products conversion in cofactor-dependent production systems. In this study, nicotinic acid (NA), the precursor of NADH and NADPH, was supplemented to the growth medium of a wild-type Clostridium sp. strain BOH3. Results showed that the addition of precursor NA to the medium led to a significant increase in the levels of NADH and NADPH. Meanwhile, a maximal cell growth rate and butanol generation rate were reached by applying a two-stage pH-shift strategy, achieving 18.7g/L butanol with a yield of 24.6% and a productivity of 0.26g/Lh. The metabolic patterns were shifted towards more reduced metabolites as reflected by higher butanol-to-acetone ratio (11%) and butanol-to-acid ratio (292%). Redistributing metabolic flux to butanol via manipulations of reducing cofactor and pH shift could become an alternative tool to realize metabolic engineering goals.
还原因子（如 NADH 和 NADPH）的可用性在提高辅因子依赖生产系统中产物转化率的效率方面起着重要作用。在本研究中，烟酰胺（NA），NADH 和 NADPH 的前体，被添加到一株野生型梭菌 BOH3 的生长培养基中。结果表明，向培养基中添加前体 NA 导致 NADH 和 NADPH 的水平显著增加。同时，通过采用两段 pH 转换策略，实现了最大的细胞生长速率和丁醇生成速率，获得了 18.7g/L 的丁醇，得率为 24.6%，生产强度为 0.26g/Lh。代谢模式向更还原的代谢物转移，表现在更高的丁醇-丙酮比（11%）和丁醇-酸比（292%）。通过操纵还原辅因子和 pH 转换来重新分配代谢通量到丁醇，可以成为实现代谢工程目标的一种替代工具。
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