1] Stem Cells and Cancer Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3052, Australia [2] Department of Medical Biology, The University of Melbourne, Parkville, Victoria 3010, Australia [3].
1] Stem Cells and Cancer Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3052, Australia [2] Department of Medicine, The University of Melbourne, Parkville, Victoria 3010, Australia [3] Department of Medical Oncology and Familial Cancer Centre, The Royal Melbourne Hospital, Parkville, Victoria 3050, Australia.
Nature. 2014 Feb 20;506(7488):322-7. doi: 10.1038/nature12948. Epub 2014 Jan 26.
The mammary epithelium undergoes profound morphogenetic changes during development. Architecturally, it comprises two primary lineages, the inner luminal and outer myoepithelial cell layers. Two opposing concepts on the nature of mammary stem cells (MaSCs) in the postnatal gland have emerged. One model, based on classical transplantation assays, postulates that bipotent MaSCs have a key role in coordinating ductal epithelial expansion and maintenance in the adult gland, whereas the second model proposes that only unipotent MaSCs identified by lineage tracing contribute to these processes. Through clonal cell-fate mapping studies using a stochastic multicolour cre reporter combined with a new three-dimensional imaging strategy, we provide evidence for the existence of bipotent MaSCs as well as distinct long-lived progenitor cells. The cellular dynamics at different developmental stages support a model in which both stem and progenitor cells drive morphogenesis during puberty, whereas bipotent MaSCs coordinate ductal homeostasis and remodelling of the mouse adult gland.
在发育过程中，乳腺上皮经历了深刻的形态发生变化。从结构上看，它由两个主要谱系组成，即内腔腔和外肌上皮细胞层。关于产后乳腺干细胞（MaSCs）性质的两种对立概念已经出现。一种基于经典移植测定的模型假设，双能 MaSCs 在协调成年腺体中的导管上皮扩张和维持中起关键作用，而第二种模型则提出，只有通过谱系追踪鉴定的单能 MaSCs 才能促进这些过程。通过使用随机多色 cre 报告基因与新的三维成像策略进行克隆细胞命运映射研究，我们为双能 MaSCs 以及不同的长寿祖细胞的存在提供了证据。不同发育阶段的细胞动力学支持这样一种模型，即干细胞和祖细胞都在青春期驱动形态发生，而双能 MaSCs 则协调导管的稳态和成年小鼠腺体的重塑。
