Visnovsky Jozef, Fiolka Roman, Kudela Erik, Slavik Pavol, Krkoska Milan, Lasabová Zora, Danko Jan
Department of Gynaecology and Obstetrics, Jessenius Faculty of Medicine, University Hospital Martin, Slovakia.
Department of Pathologic Anatomy, Jessenius Faculty of Medicine, University Hospital Martin, Slovakia.
Neuro Endocrinol Lett. 2013;34(7):675-80.
Endometrial cancer is one of the most common malignancies in women. The prevention has failed so far to develop an effective screening program and its incidence is rising in proportion to the incidence of cervical cancer. In recent years the investigation of malignancy genomics (genetic and epigenetic changes) has become the main focus of scientists because of its high sensitivity and specificity.
We conducted a prospective longitudinal study at the Dpt. of Gynaecology and Obstetrics of the Jessenius Faculty of Medicine in Martin from 2010 to 2012, in collaboration with the Institute of Pathology of the University Hospital in Martin. We analysed paraffin blocks of endometrial tissue from 123 women with endometrial cancer, hyperplasia and normal endometrial findings. By the use of bisulphidic modification technique and nested methylation-specific PCR (MSP), we analysed the methylation patterns of three genes: GSTP1, E-cad, RASSF1.
We found a statistically significant increase of methylation of the RASSF1 gene in endometrial cancer compared to simplex hyperplasia and intact endometrial tissue (p<0.001). GSTP1 and E-cad did not show any relevant methylation pattern in various endometrial lesions.
According to the results of our study, RASSF1 gene methylation could serve as a prognostic factor of endometrial carcinogenesis and could help to predict the behaviour of endometrial hyperplasia.
子宫内膜癌是女性最常见的恶性肿瘤之一。目前预防方面尚未制定出有效的筛查方案，且其发病率与宫颈癌发病率呈同步上升趋势。近年来，由于恶性肿瘤基因组学（基因和表观遗传变化）具有高敏感性和特异性，已成为科学家们的主要研究重点。
2010年至2012年，我们与马丁大学医院病理研究所合作，在马丁市耶塞纽斯医学院妇产科开展了一项前瞻性纵向研究。我们分析了123例患有子宫内膜癌、子宫内膜增生以及子宫内膜正常的女性的子宫内膜组织石蜡块。通过使用亚硫酸氢盐修饰技术和巢式甲基化特异性PCR（MSP），我们分析了三个基因（GSTP1、E-cad、RASSF1）的甲基化模式。
我们发现，与单纯性增生和正常子宫内膜组织相比，子宫内膜癌中RASSF1基因的甲基化在统计学上有显著增加（p<0.001）。在各种子宫内膜病变中，GSTP1和E-cad未显示出任何相关的甲基化模式。
根据我们的研究结果，RASSF1基因甲基化可作为子宫内膜癌发生的一个预后因素，并有助于预测子宫内膜增生的发展情况。
