Gane P, Vellayoudom J, Mollicone R, Breimer M E, Samuelsson B E, Rouger P, Gérard G, Le Pendu J, Oriol R
CNRGS, Institute CNTS, Paris, France.
Vox Sang. 1987;53(2):117-25. doi: 10.1111/j.1423-0410.1987.tb04930.x.
Eight anti-A and seven anti-B monoclonal reagents were tested in parallel, with normal and weak ABH red cell phenotypes. A whole range of different reactivity patterns was found, but by making a comparison with the results obtained using polyclonal standard reagents, two major categories of reagents were distinguished: (a) stronger and more specific reagents, and (b) reagents similar to, or weaker than, the standard polyclonal controls. The analysis of the specificity of the reagents by tissue fluorescence staining and reactivity with synthetic oligosaccharides and purified glycolipids confirmed the existence of broad and restricted specificities. Two kinds of anti-A1 reagents are described. One related to type 3/4 structures, which stains the Golgi apparatus, and another with broad anti-A specificity which cross-reacts with 'A-like' structures. The inhibition of anti-A reagents with salivas and synthetic oligosaccharide antigens gave parallel results for the secretor salivas and the difucosylated A antigens.
对8种抗A和7种抗B单克隆试剂与正常和弱ABH红细胞表型进行了平行测试。发现了一系列不同的反应模式，但通过与使用多克隆标准试剂获得的结果进行比较，区分出了两大类试剂：(a) 更强且更具特异性的试剂，以及 (b) 与标准多克隆对照相似或比其更弱的试剂。通过组织荧光染色以及与合成寡糖和纯化糖脂的反应性对试剂的特异性进行分析，证实了广泛和受限特异性的存在。描述了两种抗A1试剂。一种与3/4型结构相关，可对高尔基体进行染色，另一种具有广泛的抗A特异性，可与“A样”结构发生交叉反应。唾液和合成寡糖抗原对抗A试剂的抑制作用在分泌型唾液和二岩藻糖基化A抗原方面给出了平行结果。
