Shandong Provincial Key Laboratory of Water and Soil Conservation & Environmental Protection, Linyi University, Linyi, China.
Environ Toxicol Chem. 2014 May;33(5):1129-34. doi: 10.1002/etc.2532. Epub 2014 Mar 17.
As a persistent bioaccumulative compound, perfluorooctanoic acid (PFOA) is found in various ecosystems and receives growing attention. The acute toxicity of PFOA was tested on 2 freshwater microalgae, Chlamydomonas reinhardtii and Scenedesmus obliquus. The 96-h concentration for 50% of maximal effect (EC50) values were measured, physiological responses of the algae were investigated, and uptake of PFOA by the algae was quantified. The EC50 values for C. reinhardtii and S. obliquus were 51.9 ± 1.0 mg/L and 44.0 ± 1.5 mg/L PFOA, respectively. After 8-d exposure to PFOA ranging from 10 mg/L to 40 mg/L, the growth of C. reinhardtii was significantly inhibited, whereas that of S. obliquus was only slightly suppressed. Increases in malonaldehyde and proline levels were observed in the 2 algae when exposed to PFOA at certain concentrations, for instance, 20 mg/L and 40 mg/L, which is indicative of the trigger of a defensive mechanism. The percentage of PFOA that was adsorbed by the algae after 8-d exposure at a dosage between 5 mg/L and 20 mg/L ranged from 5.5% to 7.5%, and the uptake of PFOA by the algae exceeded 10%.
作为一种持久性的生物蓄积性化合物，全氟辛酸（PFOA）存在于各种生态系统中，受到越来越多的关注。本研究采用两种淡水微藻——莱茵衣藻（Chlamydomonas reinhardtii）和斜生栅藻（Scenedesmus obliquus）测试了 PFOA 的急性毒性。测量了 50%最大效应浓度（EC50）值，研究了藻类的生理反应，并定量了藻类对 PFOA 的吸收。C. reinhardtii 和 S. obliquus 的 EC50 值分别为 51.9±1.0mg/L 和 44.0±1.5mg/L PFOA。在 8 天暴露于 10mg/L 至 40mg/L 的 PFOA 后，C. reinhardtii 的生长受到显著抑制，而 S. obliquus 的生长仅受到轻微抑制。在一定浓度的 PFOA 暴露下，两种藻类的丙二醛和脯氨酸水平均升高，例如在 20mg/L 和 40mg/L 时，这表明触发了防御机制。在 5mg/L 至 20mg/L 剂量下暴露 8 天后，藻类吸附的 PFOA 百分比在 5.5%至 7.5%之间，藻类对 PFOA 的吸收超过 10%。
