Omairi-Nasser Amin, Galmozzi Carla V, Latifi Amel, Muro-Pastor M Isabel, Ajlani Ghada
Institut de Biologie et de Technologie de Saclay, Centre National de la Recherche Scientifique and Commissariat à l'Energie Atomique, 91191 Gif-sur-Yvette, France.
Instituto de Bioquímica Vegetal y Fotosíntesis, CSIC-Universidad de Sevilla, Américo Vespucio 49, 41092 Seville, Spain.
Microbiology (Reading). 2014 Apr;160(Pt 4):789-794. doi: 10.1099/mic.0.076042-0. Epub 2014 Jan 24.
In several cyanobacteria, petH, the gene encoding ferredoxin:NADP oxidoreductase (FNR), is transcribed from at least two promoters depending on growth conditions. Two transcripts (short and long) are translated from two different translation initiation sites, resulting in two isoforms (large and small, respectively). Here, we show that in Synechocystis PCC6803 the global transcriptional regulator NtcA activates transcription from the distal petH promoter. Modification of the NtcA-binding site prevents NtcA binding to the promoter in vitro and abolishes accumulation of the small isoform of FNR in vivo. We also demonstrate that a similar petH transcription and translation regime occurs in other cyanobacteria. The conditions under which this system operates provide hints for the function of each FNR isoform.
在几种蓝细菌中，编码铁氧化还原蛋白：NADP氧化还原酶（FNR）的基因petH，根据生长条件从至少两个启动子转录。两种转录本（短和长）从两个不同的翻译起始位点翻译，产生两种同工型（分别为大的和小的）。在这里，我们表明在集胞藻PCC6803中，全局转录调节因子NtcA激活远端petH启动子的转录。NtcA结合位点的修饰阻止NtcA在体外与启动子结合，并消除体内FNR小同工型的积累。我们还证明在其他蓝细菌中存在类似的petH转录和翻译机制。该系统运行的条件为每种FNR同工型的功能提供了线索。
