Wang James, Guo Xiaoyu, Lui Monica, Chu Pei-Ju, Yoo Jennifer, Chang Megan, Yen Yun
StemBios Technologies, Inc., Monterey Park, California, United States of America.
Board Member of the Scientific Advisory Board, StemBios Technologies, Inc., Monterey Park, California, United States of America.
PLoS One. 2014 Jan 17;9(1):e85112. doi: 10.1371/journal.pone.0085112. eCollection 2014.
Small stem cells, such as spore-like cells, blastomere-like stem cells (BLSCs), and very-small embryonic-like stem cells (VSELs) have been described in recent studies, although their multipotency in human tissues has not yet been confirmed. Here, we report the discovery of adult multipotent stem cells derived from human bone marrow, which we call StemBios (SB) cells. These isolated SB cells are smaller than 6 ìm and are DAPI+ and Lgr5+ (Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5). Because Lgr5 has been characterized as a stem cell marker in the intestine, we hypothesized that SB cells may have a similar function. In vivo cell tracking assays confirmed that SB cells give rise to three types of cells, and in vitro studies demonstrated that SB cells cultured in proprietary media are able to grow to 6-25 ìm in size. Once the SB cells have attached to the wells, they differentiate into different cell lineages upon exposure to specific differentiation media. We are the first to demonstrate that stem cells smaller than 6 ìm can differentiate both in vivo and in vitro. In the future, we hope that SB cells will be used therapeutically to cure degenerative diseases.
近年来的研究中已描述了一些小干细胞，如孢子样细胞、卵裂球样干细胞（BLSCs）和极小型胚胎样干细胞（VSELs），尽管它们在人体组织中的多能性尚未得到证实。在此，我们报告了从人骨髓中分离出的成体多能干细胞的发现，我们将其称为干细胞生物（SB）细胞。这些分离出的SB细胞小于6微米，且为DAPI阳性和Lgr5阳性（富含亮氨酸重复序列的G蛋白偶联受体5）。由于Lgr5已被确定为肠道中的干细胞标志物，我们推测SB细胞可能具有类似的功能。体内细胞追踪试验证实SB细胞可分化为三种类型的细胞，体外研究表明，在专用培养基中培养的SB细胞能够生长到6至25微米大小。一旦SB细胞附着于孔中，在暴露于特定分化培养基后它们会分化为不同的细胞谱系。我们首次证明小于6微米的干细胞能够在体内和体外分化。未来，我们希望SB细胞能够用于治疗退行性疾病。
