Zhang Hanjun, Liu Xing, Liu Youbin, Wu Yin, Li Hongxi, Zhao Chengbin, Li Huazhe, Meng Qinggang, Li Wei
Department of Orthopaedic Surgery, Harbin Medical University, Harbin, China.
Department of Orthopaedic Surgery, The First Hospital of Harbin City, Harbin, China.
PLoS One. 2014 Jan 21;9(1):e86003. doi: 10.1371/journal.pone.0086003. eCollection 2014.
The aim of the present study was to explore the potential for hematoporphyrin monomethyl ether-Sonodynamic Therapy (HMME-SDT) treatment of hypertrophic scars within rabbit ears.
60 white rabbits were randomly divided into five groups: (1) untreated controls, (2) lesioned, (3) lesioned + HMME, (4) lesioned + US (Ultrasound), and (5) lesioned +HMME-SDT. After induction of a lesion upon the ears of the rabbits, hypertrophic scars were assessed at 14, 28, 42 and 56 days post-lesion +/- treatment. Assessments consisted of visual inspection in the change of the skin, scar formation pathological morphology by hematoxylin and eosin (HE) staining technique with optical microscopy, calculation of a hypertrophic index, fibroblastic density measures, and observation of collagen changes in the scar tissue by Van Gieson's (VG)Stain along with calculation of collagen area density.
With continued HMME-SDT treatment there was a gradual improvement in all parameters over the duration of the experiment. The lesion-induced scars of rabbits receiving HMME-SDT treatment were soft, the size was reduced, hyperplasia was flat and the color pale. The fibroblasts and collagens were reduced and the collagens were light red, sparse and orderly. The hypertrophic index was reduced, since the fibroblastic density was lowered and collagen area density was decreased.
HMME is an effective sonosensitizer and the combination of HMME-SDT treatment can exert significant benefits in reducing the formation of hypertrophic scars.
本研究旨在探讨血卟啉单甲醚-声动力疗法（HMME-SDT）治疗兔耳增生性瘢痕的潜力。
60只白兔随机分为五组：（1）未治疗对照组；（2）造模组；（3）造模+HMME组；（4）造模+超声（US）组；（5）造模+HMME-SDT组。在兔耳造模后，于损伤后14、28、42和56天对增生性瘢痕进行评估（无论是否接受治疗）。评估包括皮肤变化的肉眼观察、苏木精-伊红（HE）染色技术结合光学显微镜观察瘢痕形成的病理形态、计算增生指数、测量成纤维细胞密度，以及用Van Gieson（VG）染色观察瘢痕组织中的胶原变化并计算胶原面积密度。
随着HMME-SDT治疗的持续进行，在整个实验过程中所有参数都有逐渐改善。接受HMME-SDT治疗的兔损伤诱导瘢痕质地变软，尺寸减小，增生变平且颜色变浅。成纤维细胞和胶原减少，胶原呈淡红色，稀疏且排列有序。增生指数降低，因为成纤维细胞密度降低且胶原面积密度减小。
HMME是一种有效的声敏剂，HMME-SDT联合治疗在减少增生性瘢痕形成方面可发挥显著作用。
