Tamburic Bojan, Guruprasad Supriya, Radford Dale T, Szabó Milán, Lilley Ross McC, Larkum Anthony W D, Franklin Jim B, Kramer David M, Blackburn Susan I, Raven John A, Schliep Martin, Ralph Peter J
Plant Functional Biology and Climate Change Cluster, University of Technology Sydney, Sydney, New South Wales, Australia.
Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan, United States of America.
PLoS One. 2014 Jan 20;9(1):e86047. doi: 10.1371/journal.pone.0086047. eCollection 2014.
A matrix of photobioreactors integrated with metabolic sensors was used to examine the combined impact of light and temperature variations on the growth and physiology of the biofuel candidate microalgal species Nannochloropsis oculata. The experiments were performed with algal cultures maintained at a constant 20 °C versus a 15 °C to 25 °C diel temperature cycle, where light intensity also followed a diel cycle with a maximum irradiance of 1920 µmol photons m(-2) s(-1). No differences in algal growth (Chlorophyll a) were found between the two environmental regimes; however, the metabolic processes responded differently throughout the day to the change in environmental conditions. The variable temperature treatment resulted in greater damage to photosystem II due to the combined effect of strong light and high temperature. Cellular functions responded differently to conditions before midday as opposed to the afternoon, leading to strong hysteresis in dissolved oxygen concentration, quantum yield of photosystem II and net photosynthesis. Overnight metabolism performed differently, probably as a result of the temperature impact on respiration. Our photobioreactor matrix has produced novel insights into the physiological response of Nannochloropsis oculata to simulated environmental conditions. This information can be used to predict the effectiveness of deploying Nannochloropsis oculata in similar field conditions for commercial biofuel production.
利用集成代谢传感器的光生物反应器矩阵，研究光照和温度变化对生物燃料候选微藻物种眼点拟微绿球藻生长和生理的综合影响。实验采用两种藻类培养方式，一种维持在恒定的20°C，另一种采用15°C至25°C的昼夜温度循环，光照强度也随昼夜循环变化，最大辐照度为1920 μmol光子 m(-2) s(-1)。两种环境条件下，藻类生长（叶绿素a）未发现差异；然而，代谢过程在一天中对环境条件变化的反应不同。由于强光和高温的综合作用，变温处理对光系统II造成了更大的损伤。细胞功能在中午之前和下午对条件的反应不同，导致溶解氧浓度、光系统II量子产率和净光合作用出现强烈滞后现象。夜间代谢表现不同，可能是温度对呼吸作用产生影响的结果。我们的光生物反应器矩阵为眼点拟微绿球藻对模拟环境条件的生理反应提供了新的见解。这些信息可用于预测在类似田间条件下部署眼点拟微绿球藻进行商业生物燃料生产的有效性。
