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Identification of functional regions in the C-terminal domain of Escherichia coli ribosomal protein S1 using monoclonal antibodies.

作者信息

Hahn V, Ebel J P, Stiegler P

机构信息

Laboratoire de Biochimie, Institut de Biologie Moléculaire et Cellulaire du CNRS, Strasbourg, France.

出版信息

Biochem Biophys Res Commun. 1987 Nov 30;149(1):34-9. doi: 10.1016/0006-291x(87)91601-9.

Abstract

Monoclonal antibodies specific for defined regions of E. coli ribosomal protein S1 were used in a R17 mRNA-directed protein synthesis assay to reveal functionally important sites of the protein. Two distinct sites for mRNA binding were identified in the regions 349-437 and 438-547 located in the C-terminal domain of protein S1.

摘要

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