Deletoille P, Prou O
Centre National de Transfusion sanguine, Paris.
Bull Soc Pathol Exot Filiales. 1987;80(3 Pt 2):569-80.
Development and use of murine specific monoclonal antibodies associated with an indirect immunofluorescent assay allows a rapid detection of P. falciparum antigens. This work compares the results obtained by interpretation of Giemsa staining technique on blood smears, to the use of a commercial reagent (Monofluo Kit P. falciparum). This monoclonal indirect immunofluorescent assay (I.F.I.M.) provides the clinician with a precious aid to diagnosis as well by its specificity and sensitivity, better than conventional staining techniques; particularly in pauciparasitaemia induced by prime-infestation or partial anti-malarial drug resistance.
与间接免疫荧光测定相关的鼠特异性单克隆抗体的开发和使用,使得能够快速检测恶性疟原虫抗原。这项工作将通过解读血涂片上的吉姆萨染色技术所获得的结果,与使用一种商业试剂(恶性疟原虫单荧光试剂盒)的结果进行了比较。这种单克隆间接免疫荧光测定法(I.F.I.M.)凭借其特异性和敏感性,为临床医生提供了一种宝贵的诊断辅助手段,比传统染色技术更好;特别是在初次感染或部分抗疟药物耐药引起的低原虫血症中。