哺乳动物草甸田鼠（啮齿目，仓鼠科）组成型异染色质的转录活性

Transcriptional activity of constitutive heterochromatin in the mammal Microtus agrestis (Rodentia, Cricetidae).

作者信息

Sperling K, Kalscheuer V, Neitzel H

机构信息

Institut für Humangenetik der Freien Universität Berlin.

出版信息

Exp Cell Res. 1987 Dec;173(2):463-72. doi: 10.1016/0014-4827(87)90286-2.

DOI:10.1016/0014-4827(87)90286-2

PMID:2446895

Abstract

Two cytological approaches were applied to the analysis of genetic activity in euchromatin and heterochromatin in the European field vole Microtus agrestis. The first is based on the transcriptional activity of prematurely condensed chromosomes, the second on in situ hybridization of labeled cellular RNA to metaphase chromosomes. The results show that in cultivated fibroblasts, the transcriptional activity of constitutive heterochromatin is in the same order of magnitude as that of euchromatin; its chromosomal pattern is nonrandom. It is concluded that--as has already been shown for insects and amphibians--also in mammals, constitutive heterochromatin is transcribed in its decondensed state but is genetically inactive when forming chromocenters at interphase.

摘要

采用了两种细胞学方法来分析欧洲田鼠（Microtus agrestis）常染色质和异染色质中的遗传活性。第一种方法基于早熟凝集染色体的转录活性，第二种方法基于标记的细胞RNA与中期染色体的原位杂交。结果表明，在培养的成纤维细胞中，组成型异染色质的转录活性与常染色质处于同一数量级；其染色体模式是非随机的。得出的结论是——正如已经在昆虫和两栖动物中所表明的那样——在哺乳动物中也是如此，组成型异染色质在其解聚状态下被转录，但在间期形成染色中心时是遗传无活性的。