Strnad C F, Carchman R A
Department of Pharmacology and Toxicology, Medical College of Virginia, Richmond 23298-0001.
FEBS Lett. 1987 Dec 10;225(1-2):16-20. doi: 10.1016/0014-5793(87)81123-7.
Pertussis toxin was found to serve as a mitogen in the human T lymphocyte, an effect which could be mimicked by its resolved binding component, the B oligomer. The mechanism of action of this component appeared to involve a rapid and sustained elevation of cytosolic calcium levels, as monitored by fura-2 fluorescence. The source of mobilized calcium was predominantly extracellular, suggesting that the binding of the B oligomer to the T cell plasma membrane in some way elicited calcium channel activation. Notably, the influx of calcium was not observed with cholera toxin, an AB toxin lacking mitogenic effects on the human T lymphocyte.
百日咳毒素被发现可作为人T淋巴细胞的促细胞分裂剂，其解离后的结合成分B寡聚体也能模拟这种效应。通过fura - 2荧光监测发现，该成分的作用机制似乎涉及胞质钙水平的快速持续升高。动员的钙主要来源于细胞外，这表明B寡聚体与T细胞质膜的结合以某种方式引发了钙通道的激活。值得注意的是，霍乱毒素（一种对人T淋巴细胞无促有丝分裂作用的AB毒素）未观察到钙内流现象。
