Department of Botany and Microbiology, University College, Swansea, U.K..
Planta. 1973 Mar;111(1):57-64. doi: 10.1007/BF00386735.
Nitrate reductase activity was detectable in ammonium-grown cells of Ankistrodesmus braunii after 50 minutes of nitrogen starvation. The rate of formation of nitrate reductase was stimulated by addition of nitrate and inhibited completely by cycloheximide (20 μg/ml). Nitrogen-starved cells assimilated added nitrate or nitrite rapidly and no nitrite or nitrate was detectable in either cells or culture medium from cultures subjected to nitrogen starvation. It is concluded that nitrate is not obligatory for the formation of nitrate reductase.
硝酸还原酶活性在经过 50 分钟的氮饥饿后，在氨培养的束丝藻细胞中可检测到。硝酸的添加刺激了硝酸还原酶的形成速度，而环己酰亚胺（20μg/ml）完全抑制了其形成。饥饿的细胞迅速同化添加的硝酸盐或亚硝酸盐，而在经过氮饥饿处理的培养物中，无论是细胞还是培养基中都检测不到亚硝酸盐或硝酸盐。因此可以得出结论，硝酸盐对于硝酸还原酶的形成不是必需的。
