2',4'-二羟基-6'-甲氧基-3',5'-二甲氧基查尔酮对过氧化氢诱导的肝 L02 细胞氧化应激的保护作用。
Protective effects of 2',4'-dihydroxy-6'-methoxy-3',5'-dimethylchalcone against hydrogen peroxide-induced oxidative stress in hepatic L02 cell.
机构信息
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, People's Republic of China.
出版信息
Arch Pharm Res. 2014;37(9):1211-8. doi: 10.1007/s12272-014-0334-4. Epub 2014 Jan 28.
2',4'-Dihydroxy-6'-methoxy-3',5'-dimethylchalcone (DMC) is a chalcone isolated from the buds of Cleistocalyx operculatus (Roxb.) Merr. et Perry, and the hepatoprotective effects of DMC on Kunming mice have been studied in previous study. However, the effects of DMC on hepatocyte toxicity and corresponding mechanism remain unclear. The aim of this study was to evaluate the hepatoprotective mechanism of DMC in human hepatocytes (L02) treated with H₂O₂. The results demonstrated that pretreatment with DMC effectively protected H₂O₂-induced cell viability loss, cell membrane damage (lactate dehydrogenase, nitric oxide production and caspase-3 accumulation. Besides, DMC pretreatment increased the amount of glutathione, decreased malondialdehyde and the percentage of apoptotic L02 cells compared with only H₂O₂ treated group. Taken together, these results indicated that DMC had hepatoprotective effects against H₂O₂-induced liver injury by alleviating oxidative stress and apoptosis process in L02 cells, and DMC might be a potential candidate for the intervention of liver diseases.
2',4'-二羟基-6'-甲氧基-3',5'-二甲氧基查尔酮（DMC）是从桃金娘科蒲桃属的花蕾中分离得到的查尔酮，先前的研究已经研究了 DMC 对昆明小鼠的保肝作用。然而，DMC 对肝细胞毒性的影响及其相应的机制尚不清楚。本研究旨在评估 DMC 对过氧化氢（H₂O₂）处理的人肝细胞（L02）的保肝机制。结果表明，DMC 预处理可有效保护 H₂O₂诱导的细胞活力丧失、细胞膜损伤（乳酸脱氢酶、一氧化氮产生和 caspase-3 积累。此外，与仅用 H₂O₂处理组相比，DMC 预处理增加了谷胱甘肽的含量，降低了丙二醛和凋亡 L02 细胞的百分比。综上所述，这些结果表明，DMC 通过减轻 L02 细胞中的氧化应激和细胞凋亡过程对 H₂O₂诱导的肝损伤具有保护作用，DMC 可能是干预肝脏疾病的潜在候选药物。
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