Pirvu T N, Schroeder J E, Peroglio M, Verrier S, Kaplan L, Richards R G, Alini M, Grad S
AO Research Institute Davos, Clavadelerstrasse 8, 7270, Davos, Switzerland.
Eur Spine J. 2014 Apr;23(4):745-53. doi: 10.1007/s00586-014-3198-x. Epub 2014 Jan 28.
Platelet-rich plasma (PRP) contains growth factors and creates a 3D structure upon clotting; PRP or platelet lysate (PL) might be considered for annulus fibrosus (AF) repair.
Bovine AF cells were cultured with 25% PRP, 50% PRP, 25% PL, 50% PL, or 10% FBS. After 2 and 4 days, DNA, glycosaminoglycan (GAG), and mRNA levels were analyzed. Histology was performed after injection of PRP into an AF defect in a whole disc ex vivo.
By day 4, significant increases in DNA content were observed in all treatment groups. All groups also showed elevated GAG synthesis, with highest amounts at 50% PL. Collagen I and II expression was similar between groups; aggrecan, decorin, and versican expression was highest at 25% PL. Injection of PRP into the AF defect resulted in an increased matrix synthesis.
Platelet-rich preparations increased the matrix production and cell number and may therefore be considered to promote AF repair.
富含血小板血浆（PRP）含有生长因子，凝血时会形成三维结构；可考虑使用PRP或血小板裂解物（PL）修复纤维环（AF）。
将牛AF细胞与25% PRP、50% PRP、25% PL、50% PL或10%胎牛血清（FBS）一起培养。培养2天和4天后，分析DNA、糖胺聚糖（GAG）和mRNA水平。将PRP注入离体完整椎间盘的AF缺损处后进行组织学检查。
到第4天，所有治疗组的DNA含量均显著增加。所有组的GAG合成也有所升高，50% PL组的含量最高。各组之间I型和II型胶原蛋白表达相似；聚集蛋白聚糖、核心蛋白聚糖和多功能蛋白聚糖在25% PL组的表达最高。将PRP注入AF缺损处可增加基质合成。
富含血小板的制剂可增加基质产生和细胞数量，因此可考虑用于促进AF修复。
