Research Centre, Centre Hospitalier de l'Université de Montréal (CRCHUM), 900 rue Saint Denis, Montreal, QC, Canada, H2X 0A9; Department of Mechanical Engineering, École de technologie supérieure (ÉTS), 1100 boul. Notre-Dame Ouest, Montréal, QC, Canada H3C 1K3.
Macromol Biosci. 2014 May;14(5):720-30. doi: 10.1002/mabi.201300443. Epub 2014 Jan 28.
An anti-apoptotic coating combining chondroitin sulfate (CS) and coiled-coil-based tethering of epidermal growth factor (EGF) is designed for vascular applications. The oriented tethering strategy enables to reach higher EGF surface densities compared to the commonly used random covalent grafting, while using much lower concentrations of EGF during incubation. It also significantly improves vascular smooth muscle cell (VSMC) survival and resistance to apoptosis in serum-free conditions. The comparison of CS and low-fouling carboxymethylated dextran as a sublayer for growth factors highlights the tremendous benefit of CS thanks to its selective protein resistance and good cell adhesion properties. This approach can be tuned by capturing other growth factors on CS through coiled-coil interactions.
一种结合硫酸软骨素（CS）和表皮生长因子（EGF）的螺旋线圈系缚的抗凋亡涂层，专为血管应用而设计。与常用的随机共价接枝相比，定向系缚策略能够达到更高的 EGF 表面密度，同时在孵育过程中使用低得多的 EGF 浓度。它还显著提高了血管平滑肌细胞（VSMC）在无血清条件下的存活率和抗凋亡能力。CS 和低蛋白吸附羧甲基化葡聚糖作为生长因子亚层的比较突出了 CS 的巨大优势，这要归功于其对蛋白质的选择性抵抗和良好的细胞粘附特性。通过卷曲螺旋相互作用将其他生长因子捕获到 CS 上，可以对这种方法进行调整。
