Drescher J, Verhagen W, Flik J, Stachan R
Institute of Virology, Medical School of Hannover, FRG.
Intervirology. 1987;27(4):205-17. doi: 10.1159/000149986.
Antibodies directed against strain-specific and common antigenic sites of H1N1 influenza virus hemagglutinin were tested comparatively, using monoclonal antibodies raised against strain A/Brazil/11/78 and polyclonal antibodies directed against strains A/Brazil/11/78, A/USSR/97/77, A/PR/301/54, and A/FM/1/47. The patterns of competition between antibodies for adsorption onto homologous virus indicated that the monoclonals comprised antibodies directed to each of the two strain-specific (Sa and Sb) and common antigenic sites (Ca and Cb) of virus hemagglutinin. Polyclonal strain-specific antibodies (SSA) yielded the competition patterns of mixtures of anti-Sa and anti-Sb antibodies and polyclonal common antigen antibodies (CAA) yielded those of mixtures of antibodies directed against sites Ca and Cb, indicating that the polyclonal preparations comprised a similar repertoire of antibodies, as represented by the panel of monoclonals. This conclusion was confirmed by determining, by means of equilibrium filtration, the number of epitopes per homologous virion(s) recognized by antibody preparations and their mixtures. Polyclonal SSA and CAA gave s values not significantly different from those of mixtures of the corresponding monoclonal antibodies. The strains tested were found to possess equivalent numbers of strain-specific and common epitopes per virion. The competition between antibodies was further examined in terms of the additiveness of s values they recognize in simultaneous reactions. No competition was observed for the monoclonal antibody pairs anti-Sa/anti-Ca, anti-Sa/anti-Cb and anti-Sb/anti-Cb, indicating that these antibodies combined with nonoverlapping epitopes. Polyclonal SSA and CAA yielded partial competition. The equilibrium constants (K) of comparable SSA and CAA were within the same range, and SSA and CAA did not influence their binding avidity when allowed to react simultaneously with homologous virus.
利用针对A/巴西/11/78毒株产生的单克隆抗体以及针对A/巴西/11/78、A/苏联/97/77、A/波多黎各/301/54和A/福尔摩沙/1/47毒株的多克隆抗体，对针对H1N1流感病毒血凝素的毒株特异性和共同抗原位点的抗体进行了比较测试。抗体与同源病毒吸附竞争的模式表明单克隆抗体包含针对病毒血凝素两个毒株特异性（Sa和Sb）和共同抗原位点（Ca和Cb）中每一个的抗体。多克隆毒株特异性抗体（SSA）产生了抗Sa和抗Sb抗体混合物的竞争模式，多克隆共同抗原抗体（CAA）产生了针对Ca和Cb位点的抗体混合物的竞争模式，这表明多克隆制剂包含与单克隆抗体组所代表的类似抗体库。通过平衡过滤确定抗体制剂及其混合物识别的每个同源病毒粒子的表位数量，证实了这一结论。多克隆SSA和CAA给出的s值与相应单克隆抗体混合物的s值无显著差异。发现所测试的毒株每个病毒粒子具有等量的毒株特异性和共同表位。根据抗体在同时反应中识别的s值的加和性，进一步研究了抗体之间的竞争。未观察到抗Sa/抗Ca、抗Sa/抗Cb和抗Sb/抗Cb单克隆抗体对之间的竞争，表明这些抗体结合不重叠的表位。多克隆SSA和CAA产生部分竞争。可比的SSA和CAA的平衡常数（K）在同一范围内，并且当允许SSA和CAA与同源病毒同时反应时，它们不影响其结合亲和力。
