大鼠微粒体细胞色素b5还原酶的改进分离方法。
Improved isolation of rat microsomal cytochrome b5 reductase.
作者信息
Perkins D M, Duncan J R
机构信息
Department of Chemistry and Biochemistry, Rhodes University, Grahamstown, South Africa.
出版信息
J Chromatogr. 1987 Sep 18;405:319-25. doi: 10.1016/s0021-9673(01)81773-8.
Cytochrome b5 reductase (E.C. 1.6.2.2.) has been isolated using a modification of the methods of Takesue and Omura [J. Biochem., 67 (1970) 267], Mihara and Sato [J. Biochem., 71 (1972) 725; and J. Biochem., 78 (1975) 1057]. The methodology involves the isolation of cytochrome b5 reductase from rat liver microsomes using the techniques of lysosomal solubilisation, ammonium sulphate fractionation, gel chromatography and dialysis. This modified method results in a greatly increased yield of enzyme. The purity of the isolated enzyme is shown by disc gel electrophoresis.
细胞色素b5还原酶（E.C. 1.6.2.2.）已通过对武末和大村[《生物化学杂志》，67 (1970) 267]、三原和佐藤[《生物化学杂志》，71 (1972) 725；以及《生物化学杂志》，78 (1975) 1057]方法的改进进行了分离。该方法包括使用溶酶体溶解、硫酸铵分级分离、凝胶色谱和透析技术从大鼠肝脏微粒体中分离细胞色素b5还原酶。这种改进的方法使酶的产量大幅提高。通过圆盘凝胶电泳显示了分离酶的纯度。
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