Department Veterinary Public Health and Food Safety, Faculty Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium.
Vet Microbiol. 2014 Mar 14;169(3-4):223-7. doi: 10.1016/j.vetmic.2013.12.019. Epub 2014 Jan 10.
Yersiniosis is a common bacterial zoonosis in Europe and healthy pigs are known to be the primary reservoir of human pathogenic Yersinia enterocolitica and Y. pseudotuberculosis. However, little information is available about the prevalence of these pathogens within pig batches at time of slaughter. The tonsils of 7047 fattening pigs, belonging to 100 farms, were aseptically collected immediately after evisceration in two Belgian slaughterhouses. The batch size varied between 70 and 930 pigs. On average, 70 pigs were sampled per batch. The tonsils were examined by direct plating on cefsulodin-irgasan-novobiocin (CIN) agar plates and the number of suspect Yersinia colonies was counted. Pathogenic Y. enterocolitica serotype O:3 were found in tonsils of 2009 pigs (28.5%), originating from 85 farms. The within-batch prevalence in positive farms ranged from 5.1 to 64.4%. The number of Y. enterocolitica in positive pigs varied between 2.01 and 5.98 log10 CFU g(-1) tonsil, with an average of 4.00 log10 CFU g(-1) tonsil. Y. pseudotuberculosis was found in seven farms, for which the within-batch prevalence varied from 2 to 10%. In five of these farms, both Y. enterocolitica and Y. pseudotuberculosis were simultaneously present. Human pathogenic Yersinia spp. are widespread in slaughter pig batches in Belgium as 87% of the tested batches were infected with these pathogens at the time of slaughter. The large variation of the prevalence between batches may lead to different levels of contamination of carcasses and risks for public health.
耶尔森菌病是欧洲常见的细菌性人畜共患病，健康猪被认为是人类致病性肠耶尔森菌和假结核耶尔森菌的主要储存宿主。然而，关于屠宰时猪批次中这些病原体的流行情况，信息很少。在比利时的两个屠宰场，从 100 个农场的 7047 头育肥猪中无菌采集扁桃体。批处理大小在 70 到 930 头猪之间变化。平均而言，每个批次采样 70 头猪。通过直接在头孢磺啶-碘酸钠-新生霉素（CIN）琼脂平板上进行平板划线检查扁桃体，并计算可疑耶尔森菌菌落的数量。在 2009 头猪（28.5%）的扁桃体中发现了致病性肠耶尔森菌 O:3 血清型，这些猪来自 85 个农场。阳性农场的批次内流行率范围为 5.1%至 64.4%。阳性猪中的肠耶尔森菌数量在 2.01 到 5.98 log10 CFU g(-1) 扁桃体之间变化，平均为 4.00 log10 CFU g(-1) 扁桃体。在 7 个农场中发现了假结核耶尔森菌，其批次内流行率在 2%至 10%之间变化。在其中 5 个农场中，同时存在肠耶尔森菌和假结核耶尔森菌。在比利时，屠宰猪批次中广泛存在人类致病性耶尔森氏菌，87%的受检批次在屠宰时被这些病原体感染。批次间流行率的巨大差异可能导致胴体污染程度和公共卫生风险的不同。
