Wildemann Paula, Gava Danielle, Sfaciotte Ricardo Antônio Pilegi, Melo Fernanda Danielle, Ferraz Sandra Maria, da Costa Ubirajara Maciel, Vaz Eliana Knackfuss
Centro de Ciências Agroveterinárias, Universidade do Estado de Santa Catarina, Av. Luiz de Camões 2090, Lages, SC, 88520-000, Brazil.
, EMBRAPA Suínos e Aves, BR153, Km110, Concórdia, SC, 89715-899, Brazil.
Trop Anim Health Prod. 2018 Mar;50(3):671-675. doi: 10.1007/s11250-017-1437-y. Epub 2017 Sep 29.
Yersinia enterocolitica is a foodborne pathogen and pigs are the main reservoir of it in their tonsils. As Brazil is a large producer and exporter of pork meat and information regarding this pathogen is still quite scarce, this study aimed at evaluating the direct detection of Y. enterocolitica followed by pathogenic Y. enterocolitica (PYE) determination in tonsils of slaughtered pigs. For this purpose, 400 pig tonsils were collected from 15 farms in four federally certified slaughterhouses in Southern Brazil. Initially, samples were screened using conventional PCR targeting of the 16sRNA gene, followed by multiplex PCR (mPCR) in order to detect three virulence genes (ail, yadA, and virF) and quantitative real-time PCR (qPCR) for the detection of the ail gene. One hundred and one (25.2%) of the samples tested positive for the 16sRNA gene. However, a PYE was detected in one out of the 101 Y. enterocolitica positive samples. The three virulence genes were determined by mPCR and confirmed by partial DNA sequencing. Thus, a significant occurrence of Y. enterocolitica was observed in pig tonsils from federally inspected slaughterhouses in Brazil, although the presence of pathogenic strains was quite low.
小肠结肠炎耶尔森菌是一种食源性病原体，猪是其在扁桃体中的主要宿主。由于巴西是猪肉的大型生产国和出口国，而关于这种病原体的信息仍然相当匮乏，本研究旨在评估在屠宰猪的扁桃体中直接检测小肠结肠炎耶尔森菌，随后测定致病性小肠结肠炎耶尔森菌（PYE）。为此，从巴西南部四个联邦认证屠宰场的15个农场收集了400个猪扁桃体。最初，使用针对16sRNA基因的常规PCR对样本进行筛查，随后进行多重PCR（mPCR）以检测三个毒力基因（ail、yadA和virF），并使用定量实时PCR（qPCR）检测ail基因。101个（25.2%）样本的16sRNA基因检测呈阳性。然而，在101个小肠结肠炎耶尔森菌阳性样本中仅有1个检测到PYE。通过mPCR确定了这三个毒力基因，并通过部分DNA测序进行了确认。因此，在巴西联邦检查的屠宰场的猪扁桃体中观察到小肠结肠炎耶尔森菌的大量存在，尽管致病菌株的存在相当低。
