Kang Jeong-Woo, Park Yun Sun, Lee Dong Hun, Kim Man Sub, Bak Yesol, Ham Sun Young, Park Su Ho, Kim Heejong, Ahn Joong Hoon, Hong Jin Tae, Yoon Do-Young
Department of Bioscience and Biotechnology, Bio/Molecular Informatics Center, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
College of Pharmacy and Medical Research Center, Chungbuk National University, 12 Gashin-dong, Heungduk-gu, Cheongju, Chungbuk 361-463, Republic of Korea.
Biochimie. 2014 Jun;101:248-51. doi: 10.1016/j.biochi.2014.01.013. Epub 2014 Jan 25.
IL-32 has been studied for its pleiotropic effects ranging from host immune responses to cell differentiation. Although several IL-32 isoforms have been characterized for their effects on cells, the roles of the others remain unclear. We previously reported that IL-32δ interacted with IL-32β and inhibited IL-32β-mediated IL-10 production. Thus, we performed comprehensive analyses to reveal more interactions between IL-32 isoforms in this study. We screened the interactions of 81 combinations of nine IL-32 isoforms by using a yeast two-hybrid assay, which identified 13 heterodimeric interactions. We verified these results by using reciprocal immunoprecipitation assays and reconfirmed 10 interactions, and presented the interaction network map between IL-32 isoforms. Our data suggest that IL-32 may have diverse intracellular effects through the interactions with its different isoforms.
白细胞介素-32（IL-32）因其从宿主免疫反应到细胞分化的多效性作用而受到研究。尽管已经对几种白细胞介素-32亚型对细胞的作用进行了表征，但其他亚型的作用仍不清楚。我们之前报道过，白细胞介素-32δ与白细胞介素-32β相互作用，并抑制白细胞介素-32β介导的白细胞介素-10产生。因此，在本研究中，我们进行了全面分析以揭示白细胞介素-32亚型之间更多的相互作用。我们通过酵母双杂交试验筛选了九种白细胞介素-32亚型的81种组合的相互作用，确定了13种异二聚体相互作用。我们通过相互免疫沉淀试验验证了这些结果，并再次确认了10种相互作用，并呈现了白细胞介素-32亚型之间的相互作用网络图。我们的数据表明，白细胞介素-32可能通过与其不同亚型的相互作用而具有多种细胞内效应。
