Lampe H B, Flint A, Wolf G T, McClatchey K D
Department of Otolaryngology, University of Western Ontario, London, Canada.
J Otolaryngol. 1987 Dec;16(6):371-6.
The flow cytometer is capable of analyzing a large number of cells or cell nuclei. The flow cytometer has been used to analyze the DNA content of tumor cells from squamous cell carcinomas of the upper aerodigestive tract using a modification of the Vindelov staining procedure. The main difficulty in analyzing solid tumors using flow cytometry technology is the disaggregation of the cells and the quantification of the diploid point (2N point). A set of internal standards was developed for establishing the 2N point, as well as a technique for disaggregation, which is described. A histogram characteristic of a more aggressive squamous cell carcinoma which tends to recur more quickly and more frequently than the other squamous cell carcinoma histogram types was identified. The flow cytometer proved to be a practical method of analysis allowing for quantitative measurement of DNA content from a large number of cells originating from a squamous cell carcinoma. The trend between recurrence and histogram type will require further study.
流式细胞仪能够分析大量细胞或细胞核。通过对温德洛夫染色程序进行改进，流式细胞仪已被用于分析上消化道鳞状细胞癌肿瘤细胞的DNA含量。使用流式细胞术技术分析实体瘤的主要困难在于细胞的解离以及二倍体点（2N点）的定量。开发了一组用于确定2N点的内部标准以及一种解离技术，并对此进行了描述。确定了一种侵袭性更强的鳞状细胞癌的直方图特征，这种类型的鳞状细胞癌往往比其他类型的鳞状细胞癌直方图更快、更频繁地复发。事实证明，流式细胞仪是一种实用的分析方法，可对来自鳞状细胞癌的大量细胞的DNA含量进行定量测量。复发与直方图类型之间的关系还需要进一步研究。
