Colling M, Wolfram G
Institut für Ernährungswissenschaft, Technischen Universität München, Freising-Weihenstephan, Bundesrepublik Deutschland.
Z Lebensm Unters Forsch. 1987 Oct;185(4):288-91. doi: 10.1007/BF01123033.
The total purine content and the content of RNA, DNA, nucleotides, nucleosides and free purine bases has been determined in commercial raw food. After hydrolysing food samples with acid, the total purine content is enzymatically determined as uric acid. For the determination of the nucleic acid content, a method is chosen that allows for the analysis of the composition of nucleic acids. The amount of purine bound in nucleic acids and of purine bound in nucleotides, nucleosides and free bases is very different. The content of nucleic acids is especially high in the innards of veal, pork and beef. In these samples the quantity of purine bound in nucleotides, nucleosides and bases is very small. In trout and herring, however, more purine is bound in RNA and DNA. The same is true of roe, pork and beef muscle. Peas and beans have the lowest total purine content of all the samples examined.
已测定了市售生鲜食品中的总嘌呤含量以及RNA、DNA、核苷酸、核苷和游离嘌呤碱的含量。用酸水解食品样品后,总嘌呤含量通过酶法测定为尿酸。对于核酸含量的测定,选择了一种能够分析核酸组成的方法。核酸中结合的嘌呤量与核苷酸、核苷和游离碱中结合的嘌呤量差异很大。小牛肉、猪肉和牛肉的内脏中核酸含量特别高。在这些样品中,核苷酸、核苷和碱基中结合的嘌呤量非常少。然而,在鳟鱼和鲱鱼中,更多的嘌呤结合在RNA和DNA中。鱼子、猪肉和牛肉肌肉也是如此。在所有检测的样品中,豌豆和豆类的总嘌呤含量最低。