Zou Wenjun, Wu Zhifeng, Xiang Xiaoli, Sun Song, Zhang Jie
Department of Ophthalmology, Wuxi Second People's Hospital Affiliated to Nanjing Medical University, 68 Zhongshan Road, 214002, Wuxi, People's Republic of China.
Graefes Arch Clin Exp Ophthalmol. 2014 Apr;252(4):665-72. doi: 10.1007/s00417-014-2567-9. Epub 2014 Jan 31.
Human leukocyte antigen B27 (HLA-B27)-associated uveitis is the most common reason for non-infectious uveitis. This purpose of the research was to study the expression and significance of T lymphocyte subsets and CD₄⁺ CD₂₅⁺ T regulatory (Treg) cells in peripheral blood of patients with Human leukocyte antigen B27-positive acute anterior uveitis (HLA-B27-positive AAU).
The concentrations of Th1, Th2, Th17, CD₄⁺ CD₂₅⁺and CD₄⁺ CD₂₅⁺FOXP3⁺ Treg cells in peripheral blood were tested by flow cytometry. C-reactive protein (CRP) in peripheral blood was detected by immunoturbidimetry (ITM). Spearman's rank correlation was used to analyze the relationships between the concentration of Th1, Th2, Th17, CD₄⁺ CD₂₅⁺, and CD₄⁺ CD₂₅⁺ FOXP3(+) Treg cells in peripheral blood and disease activity score and CRP content.
The ratio of both γ [interferon (IFN)-γ] (+)CD4⁺Th1 cells and CD4⁺IL-17⁺Th17 cells in peripheral blood of patients with HLA-B27-positive AAU (P = 0.041) was higher than that of the control group (P = 0.002). The concentration of CD₄⁺ CD₂₅⁺ FOXP3(+) T cells in peripheral blood of patients with AAU was lower than that of the control group (P = 0.026). The concentration of Th1 cells in peripheral blood of the patients had no correlation with disease activity score (P = 0.50) or CRP content (P = 0.383). This was also true of the concentration of Th2 cells (Disease activity score: R = 0.068, P = 0.817; CRP content: R = 0.439, P = 0.116). Th17 cell concentration positively correlated with disease activity score (R = 0.805, P = 0.001). The concentration of CD₄⁺ CD₂₅⁺ T cells showed no correlation with disease activity score (R =-0.209, P = 0.472) or CRP content (R =-0.169, P = 0.563), whereas the concentration of CD4⁺ CD25⁺ FOXP3⁺ T cells negatively correlated with disease activity score but did not correlate with CRP (R =-0.248, P = 0.392).
The peripheral blood of patients with HLA-B27-positive AAU showed a higher expression of interferon-γ and interleukin-17 cells in CD4⁺T cells, whereas CD4⁺CD25⁺FOXP3⁺ T cells displayed a lower expression of the cytokines. The balance between Th17 cells and CD4⁺ CD25⁺ FOXP3⁺ T cells may contribute to the activity of HLA-B27-positive AAU.
人类白细胞抗原B27（HLA - B27）相关葡萄膜炎是非感染性葡萄膜炎最常见的病因。本研究旨在探讨人类白细胞抗原B27阳性急性前葡萄膜炎（HLA - B27阳性AAU）患者外周血中T淋巴细胞亚群及CD₄⁺CD₂₅⁺调节性T（Treg）细胞的表达及意义。
采用流式细胞术检测外周血中Th1、Th2、Th17、CD₄⁺CD₂₅⁺及CD₄⁺CD₂₅⁺FOXP3⁺Treg细胞的浓度。采用免疫比浊法（ITM）检测外周血中C反应蛋白（CRP）。采用Spearman等级相关分析外周血中Th1、Th2、Th17、CD₄⁺CD₂₅⁺及CD₄⁺CD₂₅⁺FOXP3⁺Treg细胞浓度与疾病活动评分及CRP含量之间的关系。
HLA - B27阳性AAU患者外周血中γ干扰素（IFN）-γCD4⁺Th1细胞及CD4⁺IL - 17⁺Th17细胞的比例均高于对照组（P = 0.041，P = 0.002）。AAU患者外周血中CD₄⁺CD₂₅⁺FOXP3(+)T细胞浓度低于对照组（P = 0.026）。患者外周血中Th1细胞浓度与疾病活动评分（P = 0.50）及CRP含量（P = 0.383）均无相关性。Th2细胞浓度亦如此（疾病活动评分：R = 0.068，P = 0.817；CRP含量：R = 0.439，P = 0.116）。Th17细胞浓度与疾病活动评分呈正相关（R = 0.805，P = 0.001）。CD₄⁺CD₂₅⁺T细胞浓度与疾病活动评分（R = - 0.209，P = 0.472）及CRP含量（R = - 0.169，P = 0.563）均无相关性，而CD4⁺CD25⁺FOXP3⁺T细胞浓度与疾病活动评分呈负相关，但与CRP无相关性（R = - 0.248，P = 0.392）。
HLA - B27阳性AAU患者外周血中CD4⁺T细胞中干扰素 - γ及白细胞介素 - 17细胞表达较高，而CD4⁺CD25⁺FOXP3⁺T细胞中细胞因子表达较低。Th17细胞与CD4⁺CD25⁺FOXP3⁺T细胞之间的平衡可能与HLA - B27阳性AAU的活动有关。
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