Zhongshan Ophthalmic Center, State Key Laboratory of Ophthalmology, Sun Yat-sen University, Guangzhou, P R China.
Ocul Immunol Inflamm. 2010 Jan;18(1):38-43. doi: 10.3109/09273940903414525.
To investigate the role of Foxp3-positive regulatory T cells in the development of experimental autoimmune uveoretinitis (EAU).
B10RIII mice were immunized with 50 microg IRBP(161-180) in complete Freund's adjuvant (CFA) to induce EAU. EAU was evaluated clinically and pathologically on days 0, 7, 14, 21, and 28. Foxp3 mRNA levels were detected using reverse transcription-PCR (RT-PCR) and the frequencies of CD4(+)Foxp3(+) T cells and CD4(+)CD25(+)Foxp3(+) T cells in splenocytes were assessed by flow cytometry at the aforementioned time points.
The first clinical signs of EAU were observed on day 8-9, worsened up to day 14, and then gradually resolved. Histopathologic results showed that inflammatory signs occurred on day 7, reached their peak on day 14, and then gradually decreased. The levels of Foxp3 mRNA and the frequencies of CD4(+)Foxp3(+) T cells and CD4(+)CD25(+)Foxp3(+) T cells in splenocytes increased on day 7, reached a peak on day 14, and then maintained at a high level until day 28.
An upregulation of Foxp3 expression is induced in EAU and paralleled with disease activity, suggesting a role for this lymphocyte subpopulation in the regression of this experimental uveitis model.
研究 Foxp3 阳性调节 T 细胞在实验性自身免疫性葡萄膜炎（EAU）发生中的作用。
B10RIII 小鼠用 50μg IRBP（161-180）完全弗氏佐剂（CFA）免疫诱导 EAU。于 0、7、14、21 和 28 天评估临床和病理 EAU。采用逆转录-PCR（RT-PCR）检测 Foxp3mRNA 水平，流式细胞术检测脾细胞中 CD4（+）Foxp3（+）T 细胞和 CD4（+）CD25（+）Foxp3（+）T 细胞的频率。
EAU 的第一个临床症状于第 8-9 天观察到，至第 14 天加重，然后逐渐缓解。组织病理学结果显示炎症征象于第 7 天发生，第 14 天达高峰，然后逐渐减少。Foxp3mRNA 水平和脾细胞中 CD4（+）Foxp3（+）T 细胞和 CD4（+）CD25（+）Foxp3（+）T 细胞的频率于第 7 天升高，第 14 天达高峰，然后维持在高水平直至第 28 天。
Foxp3 表达上调诱导 EAU，与疾病活动平行，提示该淋巴细胞亚群在该实验性葡萄膜炎模型的消退中起作用。
