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[关于纤毛微星鼓藻(拉尔夫斯)过氧化物酶体中过氧化氢酶活性的证据]

[Evidence for catalase activity in peroxisomes of Micrasterias fimbriata (Ralfs)].

作者信息

Tourte M

机构信息

Cytophysiologie de la Photosynthèse, C. N. R. S., Gif sur Yvette, France.

出版信息

Planta. 1972 Mar;105(1):50-9. doi: 10.1007/BF00385163.

DOI:10.1007/BF00385163
PMID:24477702
Abstract

By ultrastructural methods, cytology, and cytochemistry it is shown that peroxysomes are present during all stages of the life cycle of the green unicellular alga Micrasterias fimbriata, cultivated on mineral medium. These organelles, surrounded by a single membrane, are in connection with endoplasmic reticulum. In full-grown cells, they are preferentially situated near chloroplasts and cell walls. The number of peroxisomes increase before cellular division and the organelles flow into the young bulge in front of the chloroplast.Application of a modified Graham and Karnosky's medium using DAB at pH 9 shows that an important activity of catalase is present not only at the level of peroxisomes but also at the level of the cell walls and certain Golgi vesicles.The topographic relations of peroxisomes with different cellular organelles and their possible functions in cell wall or mucus synthesis are discussed.

摘要

通过超微结构方法、细胞学和细胞化学研究表明,在以矿物培养基培养的绿色单细胞藻类纤毛微星鼓藻生命周期的所有阶段都存在过氧化物酶体。这些由单层膜包围的细胞器与内质网相连。在成熟细胞中,它们优先位于叶绿体和细胞壁附近。过氧化物酶体的数量在细胞分裂前增加,并且这些细胞器流入叶绿体前方的幼嫩凸起中。使用pH值为9的二氨基联苯胺(DAB)的改良格雷厄姆和卡尔诺斯基培养基显示,过氧化氢酶的重要活性不仅存在于过氧化物酶体水平,也存在于细胞壁和某些高尔基体囊泡水平。本文讨论了过氧化物酶体与不同细胞器的拓扑关系及其在细胞壁或黏液合成中的可能功能。

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本文引用的文献

1
Leaf microbodies (peroxisomes) and catalase localization in plants differing in their photosynthetic carbon pathways.叶片微体(过氧化物酶体)和过氧化氢酶在光合作用碳代谢途径不同的植物中的定位。
Planta. 1971 Jun;97(2):93-105. doi: 10.1007/BF00386757.
2
[Microbodies and diaminobenzidine reaction in the acetate flagellates Polytomella caeca and Chlorogonium elongatum].[醋酸鞭毛虫盲眼多囊藻和细长绿梭藻中的微体与二氨基联苯胺反应]
Planta. 1971 Jun;100(2):155-66. doi: 10.1007/BF00385216.
3
The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.
Protoplasma. 2009 Mar;235(1-4):57-66. doi: 10.1007/s00709-009-0031-1. Epub 2009 Feb 12.
4
The surprising complexity of peroxisome biogenesis.过氧化物酶体生物发生的惊人复杂性。
Plant Mol Biol. 1998 Sep;38(1-2):163-89.
5
The ultrastructure and cytochemistry of microbodies in Porphyridium.紫球藻中微体的超微结构和细胞化学
Protoplasma. 1974;80(1):233-44. doi: 10.1007/BF01666362.
6
Further enzymic studies and electron microscopy of the microbodies of a mutant of Chlorella vulgaris.对普通小球藻突变体微体的进一步酶学研究及电子显微镜观察。
Arch Mikrobiol. 1973;92(1):21-38. doi: 10.1007/BF00409508.
7
An ultrastructural and cytochemical characterization of microbodies in the green algae.绿藻中微体的超微结构和细胞化学特征
Protoplasma. 1975;83(3):269-95. doi: 10.1007/BF01282559.
8
Cytochemical discrimination between catalases and peroxidases using diaminobenzidine.使用二氨基联苯胺对过氧化氢酶和过氧化物酶进行细胞化学鉴别。
Histochemistry. 1975;41(4):281-312. doi: 10.1007/BF00490073.
在电子显微镜检查中,将高pH值的柠檬酸铅用作电子不透明染色剂。
J Cell Biol. 1963 Apr;17(1):208-12. doi: 10.1083/jcb.17.1.208.
4
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J Cell Biol. 1970 Jun;45(3):576-85. doi: 10.1083/jcb.45.3.576.
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8
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9
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J Cell Biol. 1969 Nov;43(2):343-53. doi: 10.1083/jcb.43.2.343.
10
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J Histochem Cytochem. 1969 Oct;17(10):675-80. doi: 10.1177/17.10.675.