检测植物中的组蛋白修饰。
Detecting histone modifications in plants.
作者信息
Song Jie, Rutjens Bas, Dean Caroline
机构信息
Cell & Developmental Biology, John Innes Centre, Imperial College London, London, SW7 2AZ, UK.
出版信息
Methods Mol Biol. 2014;1112:165-75. doi: 10.1007/978-1-62703-773-0_11.
Histone modifications play an essential role in chromatin-associated processes including gene regulation and epigenetic inheritance. It is therefore very important to quantitatively analyze histone modifications at both the single gene and whole genome level. Here, we describe a robust chromatin immunoprecipitation (ChIP) method for Arabidopsis, which could be adapted for other plant species. This method is compatible with multiple downstream applications including qPCR, tilling arrays, and high-throughput sequencing.
组蛋白修饰在包括基因调控和表观遗传继承在内的与染色质相关的过程中起着至关重要的作用。因此，在单基因和全基因组水平上对组蛋白修饰进行定量分析非常重要。在这里，我们描述了一种适用于拟南芥的可靠的染色质免疫沉淀（ChIP）方法，该方法也可适用于其他植物物种。此方法与多种下游应用兼容，包括qPCR、耕作阵列和高通量测序。
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