乳腺癌中血管内皮生长因子+405G>C、-1154G>A多态性的患病率
Prevalence of +405G>C,-1154G>A Vascular Endothelial Growth Factor Polymorphism in Breast Cancer.
作者信息
James Rani, Ramesh G, Krishnamoorthy Lakshmi, Bhagat Rahul, Chadaga Shilpa, Deshmane Vijayalaxmi, Ramaswamy Girija
机构信息
Department of Biochemistry, Kidwai Memorial Institute of Oncology, Dr. M.H. Marigowda Road, Bangalore, 560029 India.
Department of Surgical Oncology, Kidwai Memorial Institute of Oncology, Bangalore, 560029 India.
出版信息
Indian J Clin Biochem. 2014 Jan;29(1):21-8. doi: 10.1007/s12291-013-0307-2. Epub 2013 Feb 14.
Vascular endothelial growth factor (VEGF) plays an important role in the development of Breast Cancer. The aim of this study was to investigate the association of polymorphisms in the VEGF gene on prognosis of Breast Cancer patients. This study comprised 200 patients with histologically confirmed cases of Breast cancer and 200 controls. Genotyping of the VEGF gene polymorphisms at +405G>C,-1154G>A, were performed by PCR-RFLP analysis. Preoperative plasma VEGF levels were determined by ELISA. Amongst both cases and controls, the genotypic distribution of the individual SNPs were all in Hardy-Weinberg equilibrium. Mean VEGF level was significantly elevated in cases compared to controls (t = 8.248; P < 0.001). No significant association was found between +405G>C,-1154G>A VEGF polymorphism and Breast Cancer. Logistic regression analysis revealed that 405GG & 1154GG were associated with higher levels of VEGF.
血管内皮生长因子（VEGF）在乳腺癌的发展中起重要作用。本研究的目的是探讨VEGF基因多态性与乳腺癌患者预后的相关性。本研究包括200例经组织学确诊的乳腺癌患者和200例对照。通过PCR-RFLP分析对VEGF基因多态性+405G>C、-1154G>A进行基因分型。术前血浆VEGF水平通过ELISA测定。在病例组和对照组中，各单核苷酸多态性的基因型分布均符合Hardy-Weinberg平衡。与对照组相比，病例组的平均VEGF水平显著升高（t = 8.248；P < 0.001）。未发现VEGF基因多态性+405G>C、-1154G>A与乳腺癌之间存在显著关联。逻辑回归分析显示，405GG和1154GG与较高水平的VEGF相关。
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