Takemura M, Fukui H, Wada H
Department of Pharmacology II, Osaka University School of Medicine, Japan.
Biochem Biophys Res Commun. 1987 Dec 31;149(3):982-8. doi: 10.1016/0006-291x(87)90505-5.
The bindings of radioiodinated omega-conotoxin GVIA and [3H]-nitrendipine to subcellular fractions of rat brain were examined. The results indicated that omega-conotoxin binding site was mainly present in the mitochondrial fraction, whereas nitrendipine binding site was rich in the mitochondrial but also present in the post-mitochondrial fraction. Fractionation of the mitochondrial fraction on a sucrose density gradient centrifugation showed that the both binding sites were localized in the heavy synaptosomal fraction. These results strongly suggest that the N- and L-type voltage-sensitive calcium channels have different localizations.
研究了放射性碘化ω-芋螺毒素GVIA和[3H]-尼群地平与大鼠脑亚细胞组分的结合情况。结果表明，ω-芋螺毒素结合位点主要存在于线粒体组分中，而尼群地平结合位点在线粒体中含量丰富，但也存在于线粒体后组分中。通过蔗糖密度梯度离心对线粒体组分进行分级分离显示，这两个结合位点都定位于重突触体组分中。这些结果有力地表明，N型和L型电压敏感性钙通道具有不同的定位。
