L'vov V L, Iakovlev A P, Pluzhnikova G N, Lapina E B, Shashkov A S
Bioorg Khim. 1987 Sep;13(9):1256-65.
A specific acidic polysaccharide has been isolated from the Shigella boydii type 14 antigenic lipopolysaccharide after mild hydrolysis followed by chromatography on Sephadex G-50. The polysaccharide consists of the D-glucuronic acid, 2-acetamido-2-deoxy-D-glucose and D-galactose residues in the ratio 1:1:3. From the results of methylation analysis and partial acid hydrolysis, the structure of the repeating unit of the specific polysaccharide was deduced as follows: (-6DGalp alpha 1-4DGlcAp beta 1-6DGalp beta 1-4DGalp beta 1-4DGlcNAcp beta 1-)n. The 13C NMR spectra of native and carboxyl-reduced polysaccharides, as well as of oligosaccharides produced by partial acid hydrolysis fully confirmed the proposed structure. The approach was suggested to determine the type of substitution of uronic acid moieties in polysaccharide chain by use of chromato-mass-spectrometry of acetylated methyl esters of partially methylated aldonic acids. Serological characteristics of Sh. boydii LPS type 14 and its modified derivatives are discussed.
经温和水解后，通过Sephadex G - 50柱色谱法从鲍氏志贺菌14型抗原性脂多糖中分离出一种特定的酸性多糖。该多糖由D - 葡萄糖醛酸、2 - 乙酰氨基 - 2 - 脱氧 - D - 葡萄糖和D - 半乳糖残基按1:1:3的比例组成。根据甲基化分析和部分酸水解的结果，推导得出特定多糖重复单元的结构如下：(-6DGalpα1 - 4DGlcApβ1 - 6DGalpβ1 - 4DGalpβ1 - 4DGlcNAcpβ1-)n。天然多糖、羧基还原多糖以及部分酸水解产生的寡糖的13C NMR光谱充分证实了所提出的结构。有人建议采用部分甲基化醛糖酸的乙酰化甲酯的色谱 - 质谱联用技术来确定多糖链中糖醛酸部分的取代类型。文中还讨论了鲍氏志贺菌14型脂多糖及其修饰衍生物的血清学特征。
