Stephenson J A, Summers R J, Burcher E
Department of Pharmacology, University of Melbourne, Parkville, Vic., Australia.
Eur J Pharmacol. 1987 Oct 27;142(3):391-402. doi: 10.1016/0014-2999(87)90078-1.
The autoradiographic localization of [125I]Bolton-Hunter substance P (BHSP) was examined in slide-mounted sections of dog kidney and dog renal artery and vein. Biochemical characterization of the binding in sections of dog kidney, demonstrated that BHSP binds to a population of non-interacting sites with high affinity (KD = 0.11 +/- 0.02 nM, Bmax = 0.29 +/- 0.05 fmol/section). The binding was displaced by tachykinins in the order SP greater than NKA much greater than NKB, indicative of binding to NK-1 receptors. BHSP binding to dog kidney was localized over glomeruli and endothelium of intrarenal arteries. There was binding associated with the endothelium and adventitia of the renal artery but not the vein. Binding of BHSP to arcuate arteries and to the renal artery was dependent on the presence of an intact endothelium. No evidence was obtained for receptors associated with any renal tubules. These results suggest that in the dog, vasodilation, diuresis and natriuresis in response to SP may result from an action primarily on the vascular elements of the kidney.
在狗肾、狗肾动脉和静脉的载玻片切片中，对[125I]博尔顿-亨特P物质（BHSP）进行了放射自显影定位研究。对狗肾切片中结合情况的生化特性分析表明，BHSP与一群非相互作用位点以高亲和力结合（解离常数KD = 0.11±0.02 nM，最大结合容量Bmax = 0.29±0.05 fmol/切片）。速激肽能以P物质＞神经激肽A＞神经激肽B的顺序取代这种结合，表明其与NK-1受体结合。BHSP与狗肾的结合定位在肾小球和肾内动脉的内皮上。肾动脉的内皮和外膜有结合，但肾静脉没有。BHSP与弓形动脉和肾动脉的结合依赖于完整内皮的存在。未获得与任何肾小管相关受体的证据。这些结果表明，在狗中，对P物质的血管舒张、利尿和利钠反应可能主要是由于对肾脏血管成分的作用。
