Splitter G A, Eskra L, Abruzzini A F
Department of Veterinary Science, University of Wisconsin-Madison 53706.
Immunology. 1988 Jan;63(1):145-50.
The inability to demonstrate bovine herpes virus-1 (BHV-1)-specific lymphocyte responses from BHV-1-infected cattle has been a major difficulty in confirming the importance of cellular effector mechanisms during BHV-1 infection. We have examined the capacity of bovine cytolytic T-cell clones to lyse BHV-1-infected, concanavalin A-stimulated blast cells. Cytolysis was as high as 58% at an effector to target (E:T) ratio of 1:1. All cytolytic T-cell clones produced were genetically restricted in killing cells of the autologous genotype. Cytotoxic T-lymphocyte (CTL) clones were specific for BHV-1 but not related herpes viruses, i.e. BHV-2 and pseudorabies virus. These results provide evidence that cytolytic T lymphocytes have an antigen-specific role in the immune response of cattle against BHV-1, and that CTL may serve as effector cells in the identification of glycoproteins useful in recombinant vaccine preparation since only determinants of BHV-1 were recognized.
无法从感染牛疱疹病毒1型（BHV-1）的牛体内检测到针对BHV-1的特异性淋巴细胞反应，这一直是确认细胞效应机制在BHV-1感染过程中的重要性的主要困难。我们检测了牛细胞毒性T细胞克隆裂解受BHV-1感染、经刀豆蛋白A刺激的母细胞的能力。在效应细胞与靶细胞比例为1:1时，细胞溶解率高达58%。所产生的所有细胞毒性T细胞克隆在杀伤自体基因型细胞时均受到遗传限制。细胞毒性T淋巴细胞（CTL）克隆对BHV-1具有特异性，但对相关疱疹病毒，即BHV-2和伪狂犬病病毒无特异性。这些结果证明，细胞毒性T淋巴细胞在牛针对BHV-1的免疫反应中具有抗原特异性作用，并且由于仅识别BHV-1的决定簇，CTL可能作为效应细胞用于鉴定重组疫苗制备中有用的糖蛋白。
