CXCL12-G801A 多态性可调节台湾地区结直肠癌的发病风险。
CXCL12-G801A polymorphism modulates risk of colorectal cancer in Taiwan.
机构信息
Department of Pathology and Laboratory, Medicine Kaohsiung Veterans General Hospital Tainan Branch, Tainan, Taiwan ; Department of Medical Laboratory and Biotechnology Science, Kaohsiung Medical University, Kaohsiung, Taiwan.
School of Nutrition, Chung Shan Medical University, Taichung, Taiwan ; Department of Medical Research, Chung Shan Medical University Hospital, Taichung, Taiwan.
出版信息
Arch Med Sci. 2013 Dec 30;9(6):999-1005. doi: 10.5114/aoms.2013.39211. Epub 2013 Nov 29.
INTRODUCTION
The chemokine CXCL12, designated stromal cell-derived factor-1 (SDF-1), plays a significant role in many cancer metastases. Previous studies have shown that CXCL12-G801A, a single nucleotide polymorphism (SNP) in the 3' untranslated region, correlates with breast and lung cancer in Iran. The aim of this study was to evaluate the association of the gene variant CXCL12-G801A with colorectal cancer (CRC) in a Taiwanese cohort.
MATERIAL AND METHODS
In this study, we used a denaturing high performance liquid chromatography (DHPLC) method to analyze the frequencies of CXCL12-G801A polymorphic variants between CRC patients (n = 258) and healthy controls (n = 300) in Taiwan.
RESULTS
The SNP distribution was higher in CRC patients with TNM stage II (117/258) than healthy controls (52/300). We observed a significant increase in the G/A plus A/A genotype of the CXCL12-G801A polymorphism in CRC patients (45.35%) compared with healthy controls (17.33%). The analysis of allelic frequencies in both groups revealed that CRC patients have a higher frequency of A allele (23.45%) than healthy controls (8.67%). Furthermore, among older CRC patients, the frequency of the CXCL12-G801A genotype was significantly increased (p = 0.0148).
CONCLUSIONS
Our observations suggest that the CXCL12-G801A genotype may be associated with some clinical manifestations in CRC patients in Taiwan.
简介
趋化因子 CXCL12（也称为基质细胞衍生因子-1[SDF-1]）在许多癌症转移中起着重要作用。先前的研究表明，3'非翻译区中的单核苷酸多态性（SNP）CXCL12-G801A 与伊朗的乳腺癌和肺癌有关。本研究旨在评估该基因变体 CXCL12-G801A 与台湾人群结直肠癌（CRC）之间的关联。
材料和方法
在这项研究中，我们使用变性高效液相色谱法（DHPLC）分析了台湾 CRC 患者（n=258）和健康对照组（n=300）之间 CXCL12-G801A 多态性变异的频率。
结果
TNM 分期为 II 期（117/258）的 CRC 患者的 SNP 分布高于健康对照组（52/300）。与健康对照组（17.33%）相比，我们观察到 CRC 患者 CXCL12-G801A 多态性的 G/A 加 A/A 基因型显著增加（45.35%）。对两组的等位基因频率分析表明，CRC 患者的 A 等位基因频率（23.45%）高于健康对照组（8.67%）。此外，在年龄较大的 CRC 患者中，CXCL12-G801A 基因型的频率显著增加（p=0.0148）。
结论
我们的观察结果表明，CXCL12-G801A 基因型可能与台湾 CRC 患者的某些临床表现有关。
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