Yamaguchi A, Uede T, Kokai Y, Ishii Y, Kikuchi K
Department of Pathology, Sapporo Medical College.
Jpn J Cancer Res. 1987 Dec;78(12):1378-89.
This report describes the production and characterization of monoclonal antibody specific for an antigenic determinant, designated Y1 antigen, present on Jurkat cells. Mo-Y1 specifically reacts with Jurkat cells, but not with other cell lines. Immunoprecipitation and subsequent immunochemical analysis demonstrate that Mo-Y1 defines an antigen with a molecular weight of 200,000 and a pI value of 5.3 to 5.6. Rabbit antibody against affinity-purified Y1 antigen was prepared. A sequential immunoprecipitation study confirmed that Po-Y1 reacted with the same antigen molecule recognized by Mo-Y1. Interestingly, Mo-Y1 reacted only with Jurkat cells, whereas Po-Y1 reacted with various hematopoietic cells including T cells, B cells, macrophages, and granulocytes. Po-Y1 precipitated a 200,000-dalton component from Jurkat cells and T cells. In contrast, Po-Y1 precipitated a 230,000-dalton component from B cells. These data led us to examine the relationship between T200, leukocyte-common antigen and Y1-defined antigen. Sequential immunoprecipitation analysis clearly indicated that T200 antibody and Y1 antibody recognized the same antigen molecule. Peptide mapping of T200- and Mo-Y1-defined antigen digested with V8 protease demonstrated that there were two common major peptide chains, but one peptide chain was missing in Jurkat cells that was present in T200 glycoprotein of other cells. It is possible that minor molecular modification of T200 glycoprotein occurred in the process of oncogenesis of T cells and that the altered epitope of T200 glycoprotein was recognized by Mo-Y1. The Y1 antigen can be classified as a tumor-specific antigen.
本报告描述了针对Jurkat细胞上存在的一种抗原决定簇（命名为Y1抗原）的单克隆抗体的制备及特性。单克隆抗体Mo - Y1特异性地与Jurkat细胞反应，但不与其他细胞系反应。免疫沉淀及随后的免疫化学分析表明，Mo - Y1界定了一种分子量为200,000且等电点值为5.3至5.6的抗原。制备了针对亲和纯化的Y1抗原的兔抗体。连续免疫沉淀研究证实，兔抗体Po - Y1与Mo - Y1识别的同一抗原分子发生反应。有趣的是，Mo - Y1仅与Jurkat细胞反应，而Po - Y1与包括T细胞、B细胞、巨噬细胞和粒细胞在内的各种造血细胞反应。Po - Y1从Jurkat细胞和T细胞中沉淀出一个200,000道尔顿的成分。相比之下，Po - Y1从B细胞中沉淀出一个230,000道尔顿的成分。这些数据促使我们研究T200（白细胞共同抗原）与Y1界定的抗原之间的关系。连续免疫沉淀分析清楚地表明，T200抗体和Y1抗体识别同一抗原分子。用V8蛋白酶消化T200和Mo - Y1界定的抗原后的肽图谱显示，有两条共同的主要肽链，但在Jurkat细胞中缺少一条存在于其他细胞的T200糖蛋白中的肽链。有可能在T细胞肿瘤发生过程中T200糖蛋白发生了微小的分子修饰，并且Mo - Y1识别的是T200糖蛋白改变后的表位。Y1抗原可归类为肿瘤特异性抗原。
