Mendez-Otero R, Schlosshauer B, Barnstable C J, Constantine-Paton M
Department of Biology, Yale University, New Haven, Connecticut 06511.
J Neurosci. 1988 Feb;8(2):564-79. doi: 10.1523/JNEUROSCI.08-02-00564.1988.
The distribution of an epitope recognized by the monoclonal antibody JONES has been studied immunohistochemically in the developing nervous system of the rat. In the present report, we survey selected regions of the fetal, postnatal, and adult rat nervous system to test the hypothesis that JONES binding is invariably associated with neural cell migration and axon growth in the developing rat. A series of selected developmental stages extending from embryonic day (E) 9 to adult were used in this investigation. The distribution of JONES binding was examined using indirect immunofluorescence, as well as the immunogold procedure. Particular attention was paid to regions where the positions and timing of cell and axon migrations have been well described for the rat. JONES immunoreactivity first appears at E11-12, when it is localized to the lamina terminalis, the telencephalic-diencephalic junction, the midbrain, and the rhombic lip regions of the cytologically undifferentiated neural tube. In all the regions studied, during embryonic and early postnatal life, the labeling is very intense in the ventricular zone and shows a radial array in the adjacent intermediate and marginal zones. The expression of JONES epitope correlates particularly with times of cell migration in the retina, superior colliculus, cerebellum, and telencephalon and in regions undergoing neurite extension, such as the developing optic tract, the white matter of the cerebellum, the dorsal roots, the trigeminal system, and olfactory nerve. JONES binding becomes progressively restricted in the postnatal period. In the adult brain, immunoreactivity is present only in the retina and cerebellum. In the retina, JONES labeling is present in the outer plexiform layer and optic fiber layer. The labeling in the optic fiber layer extends to the optic nerve head and stops abruptly outside the orbit. In the cerebellum, JONES shows a radially oriented pattern throughout the molecular layer and delineates the cell bodies in the Purkinje cell layer. The only non-neural regions that show JONES immunoreactivity are the adrenal medulla and the kidney glomeruli. We conclude that the antigens recognized by the JONES monoclonal antibody are associated with the migration of subsets of cells and axons within the developing rat nervous system and, consequently, may play a role in conveying selectivity to these processes.
已采用免疫组织化学方法研究了单克隆抗体JONES所识别的表位在大鼠发育中的神经系统中的分布情况。在本报告中，我们对胎鼠、新生鼠和成年鼠神经系统的选定区域进行了研究，以验证JONES结合始终与发育中大鼠的神经细胞迁移和轴突生长相关这一假说。本研究采用了一系列从胚胎第9天（E9）到成年期的选定发育阶段。使用间接免疫荧光法以及免疫金法检测JONES结合的分布情况。特别关注了对大鼠细胞和轴突迁移的位置及时间已有详细描述的区域。JONES免疫反应性最早出现在E11 - 12期，此时它定位于终板层、端脑 - 间脑交界处、中脑以及细胞学上未分化的神经管的菱唇区域。在所有研究区域中，在胚胎期和出生后早期，标记物在脑室区非常强烈，并在相邻的中间区和边缘区呈放射状排列。JONES表位的表达尤其与视网膜、上丘、小脑、端脑以及经历神经突延伸的区域（如发育中的视束、小脑白质、背根、三叉神经系统和嗅神经）中的细胞迁移时间相关。JONES结合在出生后逐渐受到限制。在成体大脑中，免疫反应性仅存在于视网膜和小脑中。在视网膜中，JONES标记物存在于外网状层和视神经纤维层。视神经纤维层中的标记物延伸至视神经乳头，并在眶外突然停止。在小脑中，JONES在整个分子层呈现出放射状排列模式，并勾勒出浦肯野细胞层中的细胞体。显示JONES免疫反应性的唯一非神经区域是肾上腺髓质和肾小球。我们得出结论，JONES单克隆抗体所识别的抗原与发育中大鼠神经系统内细胞和轴突亚群的迁移相关，因此可能在为这些过程传递选择性方面发挥作用。
