Minc Nicolas
Institut Jacques Monod, CNRS UMR 7592, Paris Cedex 13, France.
Methods Cell Biol. 2014;120:215-26. doi: 10.1016/B978-0-12-417136-7.00014-8.
The mechanical properties of fungal cells influence their growth, division, morphogenesis, and invasiveness. These cells are characterized by high internal turgor pressure contained by a stiff but elastic cell wall. In here, we describe simple and versatile methods to measure forces of fungal growth, turgor pressure, and elastic moduli of fungi cell wall, using microfabricated polydimethylsiloxane wells of varying stiffness as single cell force sensors. We demonstrate the strength of this method with the rod-shape fission yeast Schizosaccaromyces pombe and highlight how it may be implemented for studying mechanical properties of other walled cells.
真菌细胞的力学特性影响其生长、分裂、形态发生和侵袭性。这些细胞的特征是具有由坚硬但有弹性的细胞壁所包含的高内部膨压。在此，我们描述了简单且通用的方法，使用具有不同刚度的微加工聚二甲基硅氧烷孔作为单细胞力传感器，来测量真菌生长力、膨压和真菌细胞壁的弹性模量。我们用杆状裂殖酵母粟酒裂殖酵母证明了该方法的优势，并强调了如何将其应用于研究其他有细胞壁细胞的力学特性。
