Sasaki K, Tanaka K, Murakami T, Ogino T, Kawasaki M, Takahashi M, Tenjin T
Dept. of Pathology, Yamaguchi Univ.
Gan No Rinsho. 1988 Jan;34(1):13-6.
Dual parameter analysis of FCM using rhodamine 123 (R 123) and propidium iodide (PI) has made it easy to estimate cell viability. PI stains only dead cells, whereas R 123 accumulates in the mitochondria of living cells which remain unstained by PI. Therefore the combination of R 123 and PI provides a more accurate estimation of cell viability than the conventional methods of dye exclusion. Cell viability has been estimated by this method in HeLa cells treated with adriamycin in vitro. The usefulness of this analysis is suggested for the evaluation of the effect of anticancer drugs on tumor cells.
使用罗丹明123（R123）和碘化丙啶（PI）对流式细胞术进行双参数分析，使得估算细胞活力变得容易。PI仅对死细胞染色，而R123积聚在活细胞的线粒体中，这些活细胞不会被PI染色。因此，与传统的染料排除法相比，R123和PI的组合能更准确地估算细胞活力。通过这种方法，已经在体外阿霉素处理的HeLa细胞中估算了细胞活力。这种分析方法对于评估抗癌药物对肿瘤细胞的作用具有一定价值。
